Phosphorus requirement of European sea bass (Dicentrarchus labrax L.) juveniles
Phosphorus requirement of European sea bass (Dicentrarchus labrax L.) juveniles
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DOI：
10.1111/j.1365-2109.2004.01059.x
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发表时间：
2004-06-04
影响因子：
2
通讯作者：
Pimentel-Rodrigues, A
中科院分区：
文献类型：
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作者：
Oliva-Teles, A;Pimentel-Rodrigues, A
进行了生长试验，估算了欧洲黑鱼幼鱼对磷的需要量。以酪蛋白为基础，配制了6种等氮等能半纯合日粮(分别为D_1、D_2、D_3、D_4、D_5和D_6)，磷含量分别为0.48%、0.65%、0.77%、0.86%、1.05%和1.25%。以磷酸氢钙为饲粮磷源。12组鱼，每组25尾，初始体重10g，在温控水循环系统中被分配到55-L水箱中。每种实验饲料都被分配给这些鱼的重复组。试验持续了10周，每周6天，每天给鱼喂两次，以达到明显的视觉饱足感。在试验结束时，饲喂饲料D1的鱼的最终体重显著低于其他组，但饲喂饲料D4的鱼除外。投喂饲料d1的鱼死亡率显著高于投喂其他饲料的鱼(除投喂饲料d6外)。饲料转化率和蛋白质效率显著低于其他饲料。除D4组外，其余各组鱼的氮素存留率(摄氮量百分比)均显著低于其他组。能量保留率(能量摄入量百分比)在不同组之间没有显著差异。在试验结束时，不同组之间的全身成分没有差异。整体磷含量平均为0.72%(以鲜重为基础)，不受饲料磷含量的显著影响。磷存留量平均增加6.1g kg(-1)体重，组间差异不显著。摄食D2和D3饲料的鱼的磷存留量(%磷摄入量)显着高于摄食较高磷水平的鱼。以单位体重/天计算，饲喂D2~D6组间磷存留量无显著差异，而磷损失随日粮磷摄入量呈线性增加。试验结果表明，饲料中磷含量为0.65%时，可满足鳕鱼幼鱼对磷的需要量。
A growth trial was conducted to estimate the phosphorus requirement of European sea bass juveniles. Six experimental isonitrogenous and isoenergetic semi-purified diets (casein based) were formulated to contain 0.48%, 0.65%, 0.77%, 0.86%, 1.05% and 1.25% phosphorus (diets D1, D2, D3, D4, D5 and D6 respectively). Dicalcium phosphate was used as dietary phosphorus source. Twelve groups of 25 fish of 10 g initial body weight were allocated to 55-L tanks in a thermoregulated water recirculating system. Each experimental diet was assigned to duplicate groups of these fish. The trial lasted for 10 weeks and fish were fed two times a day, 6 days a week, to apparent visual satiation. At the end of the trial, final weight of fish fed diet D1 was significantly lower than that of the other groups, except of fish fed diet D4. Mortality of fish fed diet D1 was significantly higher than that of fish fed the other diets, except for diet D6. Feed efficiency and protein efficiency ratios were significantly lower with diet D1 than with the other diets. Nitrogen retention (% nitrogen intake) of fish fed diet D1 was significantly lower than in the other groups, except that of fish fed diet D4. Energy retention (% energy intake) was not significantly different among groups. At the end of the trial there were no differences in whole body composition among groups. Whole body phosphorus content averaged 0.72% (on a fresh weight basis) and was not significantly affected by dietary phosphorus content. Phosphorus retention averaged 6.1 g kg(-1) weight gain and was not significantly different among groups. Phosphorus retention (% phosphorus intake) was significantly higher in fish fed diets D2 and D3 than in fish fed higher dietary phosphorus levels. Expressed per unit body weight per day, phosphorus retention was not significantly different among groups fed diets D2 to D6, while phosphorus losses linearly increased with dietary phosphorus intake. Results of this trial indicate that the phosphorus requirement of sea bass juveniles was satisfied with a diet containing 0.65% of phosphorus.