Changes in lipids of the salivary glands of the lone star tick, Amblyomma americanum, during feeding.
Changes in lipids of the salivary glands of the lone star tick, Amblyomma americanum, during feeding.
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DOI：
10.2307/3283719
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发表时间：
1993-12
期刊：
影响因子：
--
通讯作者：
M. M. Shipley-M.;J. Dillwith;A. Bowman;R. Essenberg;J. Sauer
中科院分区：
文献类型：
--
作者：
M. M. Shipley-M.;J. Dillwith;A. Bowman;R. Essenberg;J. Sauer
本文研究了美洲钝星星蜱（Amblyomma americanum）雌雄个体在取食过程中唾液腺的脂类组成。磷脂和中性脂质组分的脂肪酸量在摄食的初始阶段急剧增加，并在体重为100-250 mg的部分摄食雌性中达到峰值。在蜱取食期间，肉豆蔻酸（14：0）和棕榈酸（16：0）的百分比组成降低，但硬脂酸（18：0）、油酸（18：1）、亚油酸（18：2）和花生四烯酸（20：4）增加。花生四烯酸是类花生酸的前体，包括2-系列的花生四烯酸，从未进食的雌性蜱唾液腺中所有脂肪酸的1.3%增加到完全饱食的雌性蜱唾液腺中的8.2%。花生四烯酸被发现在甘油三酯馏分的未进食和进食处女女性，但只有在磷脂酰胆碱和磷脂酰乙醇胺从唾液腺的其他喂养雌性蜱。喂食和未喂食雄性蜱与喂食/未喂食、喂食/交配和未喂食雌性蜱之间的比较表明，喂食是唾液腺磷脂中花生四烯酸蓄积所必需的。
The lipid composition of salivary glands from male and female lone star ticks, Amblyomma americanum, was investigated at progressive stages of tick feeding. The amounts of fatty acids from both phospholipid and neutral lipid fractions increased dramatically during the initial stage of feeding and peaked in partially fed females weighing 100-250 mg. Percentage compositions of myristic (14:0) and palmitic acid (16:0) decreased, but stearic (18:0), oleic (18:1), linoleic (18:2), and arachidonic acid (20:4) increased during tick feeding. Arachidonic acid, the precursor to eicosanoids including the 2-series of prostaglandins, increased from 1.3% of all fatty acids in salivary glands from unfed female ticks to 8.2% in salivary glands from fully engorged female ticks. Arachidonic acid was found in the triglyceride fraction of unfed and fed virgin females but only in phosphatidylcholine and phosphatidylethanolamine from salivary glands of other fed female ticks. Comparisons between fed and unfed male ticks and fed/virgin, fed/mated, and unfed females demonstrate that feeding is necessary for accumulation of arachidonic acid in salivary gland phospholipids.