αII-Spectrin interacts with five groups of functionally important proteins in the nucleus
αII-Spectrin interacts with five groups of functionally important proteins in the nucleus
复制标题
DOI：
10.1016/j.cellbi.2006.06.005
复制
发表时间：
2006-11-01
影响因子：
3.9
通讯作者：
Lambert, Muriel W.
中科院分区：
文献类型：
--
作者：
Sridharan, Deepa M.;McMahon, Laura W.;Lambert, Muriel W.
Nonerythroid alpha-spectrin (alpha SpII Sigma*) is a structural protein that has been identified in the nucleus of mammalian cells and shown to be involved in DNA repair. It is also deficient in cells from the clinically diverse genetic disorder Fanconi anemia (FA). In order to get a clearer understanding of the role of alpha SpII Sigma* in DNA repair, and whether it may have other important functions in the nucleus, studies were undertaken to identify specific alpha SpII Sigma* protein binding partners in the nucleus. The results demonstrate that multiple proteins co-immunoprecipitate with alpha SpII Sigma* from nuclear extracts from normal human lymphoblastoid and HeLa cells. These can be grouped into five categories: structural proteins, proteins involved in DNA repair, chromatin remodeling proteins, FA proteins, and transcription and RNA processing factors. These studies indicate that alpha SpII Sigma* may play a role in a number of diverse and important processes in the nucleus and that a deficiency in this protein, as occurs in FA, could affect a number of critical cellular pathways., (c) 2006 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.
