A competitive dual-label time-resolved fluoroimmunoassay for the simultaneous determination of chloramphenicol and ractopamine in swine tissue
A competitive dual-label time-resolved fluoroimmunoassay for the simultaneous determination of chloramphenicol and ractopamine in swine tissue
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DOI：
10.1007/s11434-011-4412-4
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发表时间：
2011-04
影响因子：
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通讯作者：
Zhen Zhang;Jing-fu Liu;Yan Yao;G. Jiang
中科院分区：
文献类型：
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作者：
Zhen Zhang;Jing-fu Liu;Yan Yao;G. Jiang
A novel dual-label time-resolved fluoroimmunoassay method was developed for the simultaneous determination of chloramphenicol (CAP) and ractopamine (RAC) residues in 18 swine tissue samples, using anti-CAP and anti-RAC monoclonal antibodies labeled with europium (Eu3+) and samarium (Sm3+), respectively. The detection limits for CAP and RAC were 0.06 and 0.25 ng/g. The recovery from swine muscle samples was 102%–121% for CAP at spiking levels of 0.1–5 ng/g, and 69.8%–85.8% for RAC at spiking levels of 1–10 ng/g. The results obtained from the swine tissue samples using this method showed good agreement with those obtained using ELISA and GC-MS, with correlation coefficients (R) between 0.92–0.98.
