Study on Tissue Culture and Plantlet Regeneration of Agapanthus praecox ssp.Orientalis 'Big Blue'
Study on Tissue Culture and Plantlet Regeneration of Agapanthus praecox ssp.Orientalis 'Big Blue'
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发表时间：
2011
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通讯作者：
He Yue-qiu
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作者：
He Yue-qiu
以百子莲'大蓝'的芽为外植体，建立了百子莲'大蓝'的离体植株再生体系，结果表明，百子莲'大蓝'的最佳不定芽诱导培养基为MS +NAA 0.1 mg/L+6-BA 4.0 mg/L，在MS+ 6-BA 2.0 mg/L+NAA 0.2 mg/L+ GA30.1 mg/L的培养基上，百子莲'大蓝'的不定芽诱导和增殖效果较好，最佳生根培养基为1/2 MS + NAA0.1mg/L+ AC0.1g/L，生根率100%，试管苗移栽1个月后成活率达95%。
An in vitro plant regeneration system of Agapanthus praecox ssp.Orientalis 'Big Blue' was established using buds as explants.The results showed that the suitable adventitious buds inducing medium was murashige and skoog (MS)+NAA 0.1 mg/L+6-BA 4.0 mg/L.Adventitious buds were effectively generated and proliferated on MS+ 6-BA 2.0 mg/L+NAA 0.2 mg/L+GA_3 0.1 mg/L,which the multiple for multiplication was up to 5.08.The best rooting medium was 1/2MS+NAA 0.1 mg/L+AC 0.1 g/L with a rooting rate 100%.The plantlet was transplanted with a transplanting survival rates up to 95%after a month.