Using a yeast two-hybrid system to identify FTCD as a new regulator for HIF-1α in HepG2 cells
Using a yeast two-hybrid system to identify FTCD as a new regulator for HIF-1α in HepG2 cells
复制标题
使用酵母双杂交系统将 FTCD 鉴定为 HepG2 细胞中 HIF-1alpha 的新调节剂。
DOI：
10.1016/j.cellsig.2014.03.016
复制
发表时间：
2014-07-01
影响因子：
4.8
通讯作者：
Huang, Gang
中科院分区：
文献类型：
--
作者：
Yu, Zhenhai;Ge, Yingying;Huang, Gang
HIF-1 α与肝细胞癌（HCC）的病理有关。在这里，我们使用酵母双杂交系统筛选HIF-1 α相互作用伙伴的人肝脏cDNA文库。共鉴定了53个基因，其中包括福米胺转移酶环脱氨酶（FTCD），共免疫沉淀法证实了这一结果。此外，我们的数据表明HIF-1 α通过结合FTCD启动子的低氧响应元件介导了缺氧对FTCD诱导的影响。FTCD的下调降低了HIF-1 α在缺氧中的作用，增强了HepG2细胞的化学敏感性。我们的研究结果表明，FTCD和HIF信号之间的串扰促进了HCC的进展，从而暗示FTCD是HCC的治疗靶点。(C) 2014爱思唯尔公司版权所有。
HIF-1 alpha is implicated in hepatocellular carcinoma (HCC) pathologies. Here, we screened a human liver cDNA library for HIF-1 alpha-interacting partners using a yeast two-hybrid system. We identified 53 genes, including formiminotransferase cyclodeaminase (FTCD), which was confirmed by co-immunoprecipitation. Moreover, our data indicated that HIF-1 alpha mediated the effects of hypoxia on FTCD induction via binding to the hypoxiaresponsive elements of the FTCD promoter. Knockdown of FTCD reduced the effects of HIF-1 alpha in hypoxia and enhanced chemosensitivity in HepG2 cells. Our findings suggested crosstalk between FTCD and HIF signaling and promoted HCC progression, thus implicating FTCD as a therapeutic target for HCC. (C) 2014 Elsevier Inc. All rights reserved.