An investigation of how specimen dimensions affect biaxial mechanical characterizations with CellScale BioTester and constitutive modeling of porcine tricuspid valve leaflets
An investigation of how specimen dimensions affect biaxial mechanical characterizations with CellScale BioTester and constitutive modeling of porcine tricuspid valve leaflets
复制标题
使用 CellScale BioTester 和猪三尖瓣小叶的本构模型研究样本尺寸如何影响双轴机械特性
DOI：
10.1016/j.jbiomech.2023.111829
复制
发表时间：
2023
影响因子：
2.4
通讯作者：
Lee, Chung-Hao
中科院分区：
文献类型：
--
作者：
Laurence, Devin W.;Wang, Shuodao;Xiao, Rui;Qian, Jin;Mir, Arshid;Burkhart, Harold M.;Holzapfel, Gerhard A.;Lee, Chung-Hao
双轴力学表征是确定许多生物软组织的力学响应的公认方法。尽管一些计算和实验研究已经检查了实验因素（例如，夹紧与缝线安装）如何影响所获得的组织力学行为，但对样本尺寸在数据采集和后续建模中的作用知之甚少。在这项研究中，我们结合我们建立的力学表征框架与迭代的尺寸减小协议来测试的假设，即试样尺寸影响所观察到的双轴表征的力学行为。我们的研究结果表明，在四个样本尺寸范围内，三尖瓣瓣叶的峰值等双轴拉伸无显著差异。5× 4。进一步分析表明，在周向组织方向的低拉伸模量存在显著差异。这些差异导致后叶和隔叶的不同样本尺寸之间的Tong-Fung模型的本构模型参数显著不同。总的来说，我们的研究结果表明，试样尺寸在实验表征中起着重要的作用，但不一定在双轴测试过程中与商业CellScale BioTester的软组织力学行为的本构建模。
Biaxial mechanical characterizations are the accepted approach to determine the mechanical response of many biological soft tissues. Although several computational and experimental studies have examined how experimental factors (eg, clamped vs. suture mounting) affect the acquired tissue mechanical behavior, little is known about the role of specimen dimensions in data acquisition and the subsequent modeling. In this study, we combined our established mechanical characterization framework with an iterative size-reduction protocol to test the hypothesis that specimen dimensions affect the observed mechanical behavior of biaxial characterizations. Our findings indicated that there were non-significant differences in the peak equibiaxial stretches of tricuspid valve leaflets across four specimen dimensions ranging from 4. 5× 4. 5 mm to 9× 9 mm. Further analyses revealed that there were significant differences in the low-tensile modulus of the circumferential tissue direction. These differences resulted in significantly different constitutive model parameters for the Tong–Fung model between different specimen dimensions of the posterior and septal leaflets. Overall, our findings demonstrate that specimen dimensions play an important role in experimental characterizations, but not necessarily in constitutive modeling of soft tissue mechanical behavior during biaxial testing with the commercial CellScale BioTester.