Neurotrophic Effect of Adipose Tissue-Derived Stem Cells on Erectile Function Recovery by Pigment Epithelium-Derived Factor Secretion in a Rat Model of Cavernous Nerve Injury.
Neurotrophic Effect of Adipose Tissue-Derived Stem Cells on Erectile Function Recovery by Pigment Epithelium-Derived Factor Secretion in a Rat Model of Cavernous Nerve Injury.
复制标题
脂肪组织干细胞对海绵神经损伤大鼠模型中色素上皮衍生因子分泌的勃起功能恢复的神经营养作用
DOI：
10.1155/2016/5161248
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发表时间：
2016
影响因子：
4.3
通讯作者：
Deng C
中科院分区：
文献类型：
--
作者：
Chen X;Yang Q;Zheng T;Bian J;Sun X;Shi Y;Liang X;Gao G;Liu G;Deng C
旁分泌效应是干细胞治疗的主要机制。然而，这种效应的机制的细节仍然未知。本研究旨在探讨脂肪组织来源的干细胞（ADSCs）能否改善海绵体神经损伤所致的勃起功能障碍（CNIED）大鼠的勃起功能，并探讨其作用机制。海绵体内注射5-乙炔基-2-脱氧尿苷-（EdU-）标记的ADSCs后28 d，采用海绵体内压（ICP）法评价各组大鼠的勃起功能。脂肪干细胞在体外稳定分泌可检测的色素上皮衍生因子（PEDF）。双侧海绵体神经损伤（BCNI）组阴茎组织PEDF的表达在损伤后14 d开始增加，随后逐渐减少。在海绵体内注射后第28天，与磷酸盐缓冲盐水（PBS）处理组相比，ADSC组表现出显著增加的ICP。阴茎背神经神经元型一氧化氮合酶（nNOS）和S100表达增加，阴茎组织中平滑肌含量与胶原含量比值增加。此外，在ADSC组中鉴定出升高的PEDF、p-Akt和p-eNOS。这项研究表明，阴茎海绵体内注射ADSCs可以改善勃起功能，修复神经，纠正阴茎纤维化。一个潜在的机制是ADSC的PEDF分泌和随后的PI 3 K/Akt通路激活。
The paracrine effect is the major mechanism of stem cell therapy. However, the details of the effect's mechanism remain unknown. The aim of this study is to investigate whether adipose tissue-derived stem cells (ADSCs) can ameliorate cavernous nerve injury-induced erectile dysfunction (CNIED) rats and to determine its mechanism. Twenty-eight days after intracavernous injection of 5-ethynyl-2-deoxyuridine- (EdU-) labeled ADSCs, the erectile function of all the rats was evaluated by intracavernosal pressure (ICP). The ADSCs steadily secreted detectable pigment epithelium-derived factor (PEDF) in vitro. The expression of PEDF increased in the penis of the bilateral cavernous nerve injury (BCNI) group for 14 days and then gradually decreased. On day 28 after the intracavernous injection, the ADSCs group exhibited a significantly increased ICP compared with the phosphate buffered saline- (PBS-) treated group. Moreover, the neuronal nitric oxide synthase (nNOS) and S100 expression in penile dorsal nerves and the smooth muscle content to collagen ratio in penile tissues significantly increased. Furthermore, elevated PEDF, p-Akt, and p-eNOS were identified in the ADSCs group. This study demonstrated that intracavernous injection of ADSCs improved erectile function, repaired the nerve, and corrected penile fibrosis. One potential mechanism is the PEDF secretion of ADSCs and subsequent PI3K/Akt pathway activation.