Toxicokinetics and toxicological effects of single oral dose of fumonisin B1 containing Fusarium verticillioides culture material in weaned piglets
Toxicokinetics and toxicological effects of single oral dose of fumonisin B1 containing Fusarium verticillioides culture material in weaned piglets
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DOI：
10.1016/j.cbi.2010.03.025
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发表时间：
2010-05-14
影响因子：
5.1
通讯作者：
Correa, B.
中科院分区：
文献类型：
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作者：
Dilkin, P.;Direito, G.;Correa, B.
通过临床、病理、生化和鞘脂分析，研究了含有伏马菌素 B(1) (FB(1)) 的培养材料在雄性断奶仔猪中的毒代动力学和毒理学效应。动物接受单次口服剂量 5 mg FB(1)/kg 体重。从轮枝镰刀菌培养材料中获得。通过 HPLC 在每隔 1 小时至 6 小时以及每隔 12 小时至 96 小时收集的血浆中检测到 FB(1)。对 96 小时内粪便和尿液中消除的 FB(1) 以及中毒后 96 小时收集的肝脏样本中消除的 FB(1) 进行定量。在实验开始和结束时采集血样，测定血清酶活性、总胆红素、胆固醇、二氢鞘氨醇（Sa）、鞘氨醇（So）和Sa/So比率。给药后30分钟至36小时内在血浆中检测到FB(1)。 2小时后观察到FB(1)浓度最高，平均浓度为282μg/ml。给药后75分钟至41小时之间，尿液中仅检测到总FB(1)的0.93%，在8后24小时的间隔期间观察到最高平均浓度(561μg/ml)。给药后 8 至 84 小时内排出的粪便中检测到约 76.5% 的 FB(1)，其中在 8 至 24 小时内观察到最高水平。考虑到生化参数，仅胆固醇、碱性磷酸酶和天冬氨酸转氨酶活性显着增加。在血浆和尿液中，最高的 Sa 和 Sa/So 比率分别在 12 小时和 48 小时获得。 (C) 2010 Elsevier Ireland Ltd. 保留所有权利。
Toxicokinetics and the toxicological effects of culture material containing fumonisin B(1) (FB(1)) were studied in male weaned piglets by clinical, pathological, biochemical and sphingolipid analyses. The animals received a single oral dose of 5 mg FB(1)/kg of body weight. obtained from Fusarium verticillioides culture material. FB(1) was detected by H PLC in plasma collected at 1-h intervals up to 6 h and at 12-h intervals up to 96 h. FB(1) eliminated in feces and urine was quantified over a 96-h period and in liver samples collected 96 h post-intoxication. Blood samples were obtained at the beginning and end of the experiment to determine serum enzyme activity, total bilirubin, cholesterol, sphinganine (Sa), sphingosine (So) and the Sa/So ratio. FB(1) was detected in plasma between 30 min and 36 h after administration. The highest concentration of FB(1) was observed after 2 h, with a mean concentration of 282 mu g/ml. Only 0.93% of the total FB(1) was detected in urine between 75 min and 41 h after administration, the highest mean concentration (561 mu g/ml) was observed during the interval after 8 at 24 h. Approximately 76.5% of FB(1) was detected in feces eliminated between 8 and 84 h after administration, with the highest levels observed between 8 and 24 h. Considering the biochemical parameters, a significant increase only occurred in cholesterol, alkaline phosphatase and aspartate aminotransferase activities. In plasma and urine, the highest Sa and Sa/So ratios were obtained at 12 and 48 h, respectively. (C) 2010 Elsevier Ireland Ltd. All rights reserved.