Lysosomal enzymes in inflammatory synovial effusions.
Lysosomal enzymes in inflammatory synovial effusions.
复制标题
炎性滑液积液中的溶酶体酶。
作者：
N. Hadler;J. Spitznagel;R. Quinet
对28例炎症性和9例非炎症性滑液中的几种中性粒细胞溶酶体组分进行了免疫化学和酶分析。乳铁蛋白、髓过氧化物酶和酶测定的溶菌酶的数量与中性粒细胞计数是协变的。用合成底物测定类凝乳杆菌阳离子蛋白(组织蛋白酶G)和弹性蛋白酶的酶活性，以及免疫化学测定的溶菌酶，与中性粒细胞计数无关。尽管后一种检测方法是用人中性粒细胞溶酶体成分开发和标准化的，但它们测量的是炎性滑膜积液中不同的活性。如果以弹性蛋白为底物，则未检测到弹性蛋白酶。不管这些酶的来源是什么，它们的浓度与获取液体的关节的放射破坏程度之间存在负相关。关节液中溶解的溶酶体酶不太可能主要参与软骨破坏。
The concentrations of several polymorphonuclear neutrophilic lysosomal constituents were quantitated by immunochemical and enzymatic assays in 28 inflammatory and 9 noninflammatory synovial fluids. The quantities of lactoferrin, myeloperoxidase, and enzymatically determined lysozyme were covariate with the neutrophil count. Enzymatic activities measured with synthetic substrates developed for the assay of chymotryptic-like cationic protein (cathepsin G) and elastase, along with immunochemically determined lysozyme, were independent of the neutrophil count. Although the latter assays were developed and standardized with human neutrophilic lysosomal constituents, they measure different activities in inflammatory synovial effusions. No elastase was detected if elastin was used as the substrate. Regardless of the source of the enzymes, there was a negative correlation between their concentration and the degree of radiographic destruction of the joint from which the fluid was obtained. Lysosomal enzymes in solution in synovial fluid are not likely to be primarily involved in cartilage destruction.