Is there a channel for glucose in the plasma membrane ofSaccharomyces cerevisiae?
Is there a channel for glucose in the plasma membrane ofSaccharomyces cerevisiae?
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酿酒酵母质膜中是否存在葡萄糖通道？
DOI：
10.1007/bf02818998
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发表时间：
2008
影响因子：
2.6
通讯作者：
G. F. Fuhrmann
中科院分区：
文献类型：
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作者：
B. Völker;H. Martin;D. Michaelis;G. F. Fuhrmann
为了进一步阐明酿酒酵母的葡萄糖摄取机制，我们研究了高亲和力和极低亲和力葡萄糖转运过程的活化能。在2%葡萄糖环境中生长的DFY1细胞，分别在10、15、20和25~时测定不同生长阶段的初始葡萄糖摄取。采用具有一个Michaelis-Menten和一个一阶项的模型，采用计算机辅助非线性回归分析了输运动力学。通过将该模型与两个Michaelis-Menten项的模型进行比较，我们发现第一个模型明显更好。因此我们只使用第一个模型。在(nmol/min)/mg湿质量中得到的V m值和(laL/min)/mg湿质量中得到的一阶常数根据Arrhenius曲线绘制，计算活化能。指数生长早期、中期、晚期和平稳生长早期的DFY1细胞的活化能分别为66.2+ 7.1、56.5+ 4.2、63.2•3.3和56.5+ 2.9 kJ/mol。这些激活
In order to clarify further the mechanism of glucose uptake in Saccharomyces cerevisiae we have investigated the activation energies for the high and very low affinity glucose transport processes. Initial glucose uptake was measured at 10, 15, 20 and 25~ in DFY1 cells grown in 2% glucose to different growth phases. The transport kinetics were analyzed by computer-assisted nonlinear regression using a model with one Michaelis-Menten and one first-order term. By comparing this model with a model with two Michaelis-Menten terms we found that the first model was significantly better. Therefore we only used the first model.The values obtained for V m in (nmol/min)/mg wet mass and for the first-order constant in (laL/min)/mg wet mass were plotted according to Arrhenius to calculate the activation energies. DFY1 cells from the early, middle and late exponential and the early stationary growth phase showed activation energies of 66.2+ 7.1, 56.5+ 4.2, 63.2• 3.3 and 56.5+ 2.9 kJ/mol derived from the Vm-values. These activation
Kamaba,Y.；Arita,Y.；Ogata,S.；Muramatsu,H.；Muramatsu,T.：Eur.J.Biochem.(1987)
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