Practical synthetic route to functionalized rhodamine dyes
Practical synthetic route to functionalized rhodamine dyes
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DOI：
10.1021/ol035135z
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发表时间：
2003-09-04
期刊：
影响因子：
5.2
通讯作者：
Francis, MB
中科院分区：
文献类型：
--
作者：
Nguyen, T;Francis, MB
[图表]开发了一种合成功能化罗丹明衍生物的有效方法。数克数量的这些水溶性荧光团可以由廉价的前体制备并纯化，无需使用色谱法。通过后续反应安装了一系列与蛋白质反应的官能团，为生物分子修饰提供了材料。对于多色应用，已经开发出固相纯化策略来提供具有广泛光谱特性的罗丹明衍生物。
[GRAPHICS]An efficient method for the synthesis of functionalized rhodamine derivatives has been developed. Multigram quantities of these water-soluble fluorophores can be prepared from inexpensive precursors and purified without the use of chromatography. A series of protein-reactive functional groups has been installed through subsequent reactions, providing materials for biomolecule modification. For multicolor applications, a solid-phase purification strategy has been developed to afford rhodamine derivatives possessing a wide range of spectral properties.