Saccharomyces boulardii inhibits inflammatory bowel disease by trapping T cells in mesenteric lymph nodes
Saccharomyces boulardii inhibits inflammatory bowel disease by trapping T cells in mesenteric lymph nodes
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DOI：
10.1053/j.gastro.2006.10.001
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发表时间：
2006-12-01
期刊：
影响因子：
29.4
通讯作者：
Groux, Herve
中科院分区：
文献类型：
--
作者：
Dalmasso, Guillaume;Cottrez, Francoise;Groux, Herve
背景与目的：布氏酵母菌是一种治疗腹泻的非致病性酵母。采用小鼠炎症性肠病模型，分析S对炎症性肠病的抗炎作用。方法：以淋巴细胞转移型SCID小鼠为模型，灌胃给予S灌胃。监测体重减轻和结肠炎症状态。测定结肠组织核因子-kappaB活性。采用酶联免疫吸附试验检测结肠和肠系膜淋巴结中干扰素(IFN)γ产生的CD4(+)T细胞，并进行全面的逆转录聚合酶链式反应(RT-PCR)分析。最后，我们分析了细胞在体外和体内的迁移机制。结果：S补肾活血方能抑制肠易激综合征。S诱导肠系膜淋巴结内产生干扰素-γ的辅助性T细胞在肠系膜淋巴结内聚集，这与结肠内T细胞的数量减少和干扰素-γ的产生有关。在正常BALB/c小鼠中也观察到了S治疗对肠系膜淋巴结细胞聚集的影响，涉及到一种酵母分泌产物改变了淋巴结内皮细胞的粘附性。结论：S通过特异性改变聚集在肠系膜淋巴结中的T细胞的迁移行为，发挥独特的抗炎作用。因此，S治疗可抑制炎症结肠组织中辅助性T细胞I细胞的浸润，抑制促炎细胞因子产生所致炎症反应的放大。以上结果提示，S布地尔滴丸可能对肠易激综合征有一定的治疗作用。
Background & Aims: Saccharomyces boulardii is a nonpathogenic yeast used for treatment of diarrhea. We used a mice model of inflammatory bowel disease (IBD) to analyze the effects of S boulardii on inflammation. Methods: Lymphocyte-transferred SCID mice, displaying IBD, were fed daily with S boulardii. Weight loss and inflammatory status of the colon were monitored. Nuclear factor-kappa B activity was assessed in the colon. The CD4(+) T-cell production of interferon (IFN) gamma was evaluated by enzyme-linked immunosorbent assay, and a comprehensive reverse- transcription polymerase chain reaction (RT-PCR) analysis for both colon and mesenteric lymph nodes was performed. Finally, we analyzed cell migration mechanisms in vitro and in vivo. Results: S boulardii treatment inhibits IBD. S boulardii induces an accumulation of IFN-gamma-producing T-helper I cells within the mesenteric lymph nodes correlated with a diminution of CD4(+) T-cell number and IFN-gamma production by CD4+ T cells within the colon. The influence of S boulardii treatment on cell accumulation in mesenteric lymph nodes was also observed in normal BALB/c mice and involves modifications of lymph node endothelial cell adhesiveness by a yeast secretion product. Conclusions: S boulardii has a unique action on inflammation by a specific alteration of the migratory behavior of T cells, which accumulate in mesenteric lymph nodes. Therefore, S boulardii treatment limits the infiltration of T-helper I cells in the inflammed colon and the amplification of inflammation induced by proinflammatory cytokines production. These results suggest that S boulardii administration may have a beneficial effect in the treatment of IBD.