A single gene encoding the fiber is responsible for variations in virulence in the fowl adenoviruses
A single gene encoding the fiber is responsible for variations in virulence in the fowl adenoviruses
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DOI：
10.1128/jvi.70.8.5115-5122.1996
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发表时间：
1996-08-01
影响因子：
5.4
通讯作者：
Sheppard, M
中科院分区：
文献类型：
--
作者：
Pallister, J;Wright, PJ;Sheppard, M
产生了跨型重组鸡腺病毒(BEPS)，以确定病毒基因组中与毒力有关的区域。重组子产生两个8型有利的血清，轻度毒力的CFA 3和超强毒力的CFA 40。用这两种病毒基因组中的限制性内切酶片段转染原代鸡肾细胞。这些重组体的毒力测试定位于导致毒力差异的区域，基因组的8.4kb片段位于kb 26.6和35.0之间。根据实验室已部分鉴定的10型FAV的现有数据，该基因组片段包含三个已知身份的基因(100K、33K和pVIII)和一个介于kb31和35之间的编码潜力未知的区域(尽管该信息随后可用于1型FAV Celo)。因此，对kb 30.5-34.5之间的区域进行了测序。结果显示，该未知区域在右链上编码了一个纤维基因，在左链上编码了几个身份未知的小开放阅读框。进一步构建了在4-kb片段内含有明确交换的重组病毒，毒力测试表明该纤维是导致CFA 40和CFA 3毒力差异的原因。
Intertypic recombinant fowl adenoviruses (FAVs) were generated to determine regions of the viral genome involved in virulence. Recombinants were produced with two serotype 8 FAVs, mildly virulent CFA 3 and hypervirulent CFA 40. Restriction endonuclease fragments from the genomes of the two FAVs were used to transfect primary chicken kidney cells. Virulence testing of these recombinants located the region responsible for differences in virulence to an 8.4-kb fragment of the genome located between kb 26.6 and 35.0. According to data available for a serotype 10 FAV that had been partially characterized in the laboratory, this segment of the genome contained three genes of known identity (100K, 33K, and pVIII) and a region between kb 31 and 35 with unknown coding potential (although this information subsequently became available for a serotype 1 FAV, CELO). Therefore, the region between kb 30.5 and 34.5 was sequenced. The results revealed that the unknown region encoded a fiber gene on the right strand and several small open reading frames of unknown identity on the left strand. Further recombinant viruses containing defined exchanges within the 4-kb fragment were constructed, and virulence testing of these viruses indicated that the fiber was responsible for differences in virulence for CFA 40 and CFA 3.