[Application of multiplex PCR in genotyping of hepatitis B virus prevailing in Guangdong Province of China].
[Application of multiplex PCR in genotyping of hepatitis B virus prevailing in Guangdong Province of China].
复制标题
多重PCR在广东省乙型肝炎病毒基因分型中的应用
DOI：
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发表时间：
2002
期刊：
影响因子：
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通讯作者：
K. Luo
中科院分区：
文献类型：
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作者：
Jie Yang;Lin Dai;Ya;Shou‐Chang Yang;Yan;K. Luo
目标
建立一种便捷的多重 PCR 乙型肝炎病毒 (HBV) 基因分型方法。
方法
对GenBank中不同基因型的114条HBV DNA完整核苷酸序列进行比对，鉴定出基因型特异性序列，并根据每个基因型设计6对引物。使用这些引物进行后续的 HBV 基因分型，将这些引物单独或与其他引物一起添加到多重 PCR 反应管中，并根据扩增 DNA 的长度确定 HBV 基因型。
结果
多重PCR的基因分型结果与Lindh建立的PCR-限制性片段长度多态性产生的结果一致。本研究发现，广州市周边地区的乙肝病毒携带者中，B基因型约占45%，C基因型约占38.75%，D基因型约占16.75%。
结论
这种多重PCR方法简单、方便、更具差异性。
OBJECTIVE
To establish a convenient method for the genotyping of hepatitis B virus (HBV) using multiplex PCR.
METHOD
Based on the alignment of 114 complete nucleotide sequences of HBV DNA belonging to different genotypes, acquired from the GenBank, genotype-specific sequences were identified according to which 6 pairs of primers were designed corresponding to each genotype. Subsequent genotyping of HBV was performed using these primers that were added, either alone or in conjunction with others, into a multiplex PCR reaction tube, and HBV genotype was determined according to the length of amplified DNA.
RESULT
The genotyping result of multiplex PCR was consistent with that produced by PCR- restriction fragment length polymorphism as established by Lindh. We found in this study that among the HBV carriers in the vicinities Guangzhou of City, about 45% belonged to B genotype, 38.75% to C genotype and 16.75% to D genotype.
CONCLUSION
This multiplex PCR method is simple, convenient and more differential.