Efficacy of a Larger Version of the Hybrid Artificial Liver Support System Using a Polyurethane Foam/Spheroid Packed-Bed Module in a Warm Ischemic Liver Failure Pig Model for Preclinical Experiments
Efficacy of a Larger Version of the Hybrid Artificial Liver Support System Using a Polyurethane Foam/Spheroid Packed-Bed Module in a Warm Ischemic Liver Failure Pig Model for Preclinical Experiments
复制标题
使用聚氨酯泡沫/球体填充床模块的大型混合人工肝支持系统在热缺血性肝衰竭猪模型中的临床前实验效果
作者：
Y. Yamashita;M. Shimada;E. Tsujita;K. Shirabe;H. Ijima;K. Nakazawa;R. Sakiyama;J. Fukuda;K. Funatsu;K. Sugimachi
我们报道了聚氨酯泡沫填充床培养肝细胞球体系统作为混合型人工肝支持系统（PUF-HALSS）的有用性。本研究的目的是详细评价含2 × 1010个猪肝细胞的较大版本PUF-HALSS在热缺血性肝衰竭猪中临床使用时对肝功能血清参数的有效性。将体重为25 kg的热缺血性肝衰竭猪分为两组：（1）对照组（n = 3），其中每只猪连接至不含肝细胞的PUF-HALSS，和（2）HALSS组（n = 3），其中每只猪连接至PUF-HALSS。在HALSS组，血氨的增加被完全抑制，血乳酸水平被显著抑制。HALSS组的Fisher比率保持得更好，总胆汁酸、甘氨鹅去氧胆酸和牛磺鹅去氧胆酸的增加显著受到抑制。HALSS组的血清肌酐水平显著较低，血糖水平显著较高。两组血清肿瘤坏死因子- a水平均未升高。总之，较大版本的PUF-HALSS作为热缺血性肝衰竭猪的肝脏支持系统显示出许多优势。
We have reported the usefulness of a polyurethane foam packed-bed culture system of hepatocyte spheroids as a hybrid artificial liver support system (PUF-HALSS). The aim of this study was to evaluate in detail the efficacy in serum parameters regarding the liver function of a larger version of the PUF-HALSS containing 2 × 1010 porcine hepatocytes for clinical use in warm ischemic liver failure pigs. Warm ischemic liver failure pigs weighing 25 kg were divided into two groups: (1) a control group (n = 3), in which each pig was attached to a PUF-HALSS without hepatocytes, and (2) a HALSS group (n = 3), in which each pig was attached to a PUF-HALSS. In the HALSS group, the increase of blood ammonia was completely suppressed and blood lactate levels were significantly suppressed. The Fisher's ratio was better maintained, and the increase of total bile acid, glycochenodeoxycholic acid, and taurochenodeoxycholic acid was significantly suppressed in the HALSS group. Serum creatinine levels were significantly lower, and blood glucose levels were significantly higher in the HALSS group. Serum levels of tumor necrosis factor- a were not elevated in either group. In conclusion, the larger version of the PUF-HALSS demonstrated many advantages as a liver support system in warm ischemic liver failure pigs.
生物人工肝治疗严重肝衰竭的临床经验。
影响因子：
9
作者：
Watanabe,FD;Mullon,CJ;Hewitt,WR;Arkadopoulos,N;Kahaku,E;Eguchi,S;Khalili,T;Arnaout,W;Shackleton,CR;Rozga,J;Solomon,B;Demetriou,AA
通讯作者：
Demetriou,AA