Complete genome sequence of Vibrio campbellii strain 20130629003S01 isolated from shrimp with acute hepatopancreatic necrosis disease.
Complete genome sequence of Vibrio campbellii strain 20130629003S01 isolated from shrimp with acute hepatopancreatic necrosis disease.
复制标题
DOI：
10.1186/s13099-017-0180-2
复制
发表时间：
2017
期刊：
影响因子：
4.2
通讯作者：
Huang J
中科院分区：
文献类型：
--
作者：
Dong X;Wang H;Zou P;Chen J;Liu Z;Wang X;Huang J
坎氏弧菌广泛分布于海洋环境中，是虾、鱼、软体动物等水生生物的重要病原体。已经报道了携带pirAB vp基因的坎氏弧菌分离株，其引起急性肝胰腺坏死病（AHPND）。引起AHPND的坎氏弧菌（V. campbellii）的全基因组尚未有报道。为了在基因组水平上扩展我们对VCAHPND发病机制的理解，对从患有AHPND的虾中分离的V. campbellii 20130629003 S 01的基因组进行测序和分析。使用具有单分子实时测序的PacBio RSII平台生成V. campbellii 20130629003 S 01的完整基因组序列。20130629003 S 01菌株由两条环状染色体（1号染色体中的3，621，712 bp和2号染色体中的2，245，751 bp）和70，066、204，531、143，140和86，121 bp的四个质粒组成。该基因组包含5855个蛋白质编码基因、134个tRNA基因和37个rRNA基因。20130629003 S 01与其他参比菌株的核苷酸同源性平均值为97.46%，表明二者亲缘关系较近。我们在此提供的V. campbellii 20130629003 S 01的基因组序列及其与其他V. campbellii菌株的比较分析对于更好地理解VCAHPND的基因组特征非常重要。本文的在线版本（doi：10.1186/s13099-017-0180-2）包含补充材料，可供授权用户使用。
Vibrio campbellii is widely distributed in the marine environment and is an important pathogen of aquatic organisms such as shrimp, fish, and mollusks. An isolate of V. campbellii carrying the pirAB vp gene, causing acute hepatopancreatic necrosis disease (AHPND), has been reported. There are no previous reports about the complete genome of V. campbellii causing AHPND (VCAHPND). To extend our understanding of the pathogenesis of VCAHPND at the genomic level, the genome of V. campbellii 20130629003S01 isolated from a shrimp with AHPND was sequenced and analysed. The complete genome sequence of V. campbellii 20130629003S01 was generated using the PacBio RSII platform with single molecule, real-time sequencing. The 20130629003S01 strain consists of two circular chromosomes (3,621,712 bp in chromosome 1 and 2,245,751 bp in chromosome 2) and four plasmids of 70,066, 204,531, 143,140, and 86,121 bp. The genome contains a total of 5855 protein coding genes, 134 tRNA genes and 37 rRNA genes. The average nucleotide identity value of 20130629003S01 and other reference V. campbellii strains was 97.46%, suggesting that they are closely related. The genome sequence of V. campbellii 20130629003S01 and its comparative analysis with other V. campbellii strains that we present here are important for a better understanding of the genomic characteristics of VCAHPND. The online version of this article (doi:10.1186/s13099-017-0180-2) contains supplementary material, which is available to authorized users.