RIBOSOMAL-RNA SEQUENCE SHOWS PNEUMOCYSTIS-CARINII TO BE A MEMBER OF THE FUNGI
RIBOSOMAL-RNA SEQUENCE SHOWS PNEUMOCYSTIS-CARINII TO BE A MEMBER OF THE FUNGI
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DOI：
10.1038/334519a0
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发表时间：
1988-08-11
期刊：
影响因子：
64.8
通讯作者：
SOGIN, ML
中科院分区：
文献类型：
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作者：
EDMAN, JC;KOVACS, JA;SOGIN, ML
卡氏肺孢子虫（Pneumocystis cariniipacillonia）是AIDS中最常见的机会性感染1，并且在这些和其他免疫功能低下的患者中占显著的发病率和死亡率2。卡氏肺孢子虫（Pneumocystis cariniipacillonia）是一种分类不确定的真核微生物，可以感染许多哺乳动物宿主。从一个小的，单细胞的“滋养体”发展成一个包含八个“子孢子”的“包囊”，它的生命周期表面上类似于原生动物和真菌。形态学和超微结构观察使一些研究人员得出结论，该生物体是一种原生动物3 -6，而其他人则认为它更像真菌7 -9。系统发生关系可以从大分子序列的比较中推断10。小亚基核糖体RNA（16 S样rRNA）非常适合于此目的，因为它们在所有生物体中具有相同的功能，并且包含足够的信息来估计近距离和远距离的进化关系。基于这种比较的系统发育框架揭示了植物、动物和真菌谱系与原生动物谱系的多样性不同11。本文对肺孢子虫16 S-like rRNA的系统发育分析表明，它是真菌的一个成员。
Pneumocystis cariniipneumonia is the most common opportunistic infection in AIDS1, and accounts for significant morbidity and mortality in these and other immunocompromised patients2.P. cariniiis a eukaryotic microorganism of uncertain taxonomy that can infect numerous mammalian hosts. Developing from a small, unicellular 'trophozoite' into a 'cyst' containing eight 'sporozoites', its life cycle superficially resembles those seen both in the Protozoa and Fungi. Morphological and ultrastructural observations have lead some investigators to conclude that the organism is a protozoan3–6, while others have felt that it more closely resembles a fungus7–9. Phylogenetic relationships can be inferred from comparisons of macromolecular sequences10. Small subunit ribosomal RNAs (16S-like rRNAs) are well-suited for this purpose because they have the same function in all organisms and contain sufficient information to estimate both close and distant evolutionary relationships. Phylogenetic frameworks based upon such comparisons reveal that the plant, animal and fungal lineages are distinct from the diverse spectrum of protozoan lineages11. In this letter, phylogenetic analysis ofPneumocystis16S-like rRNA demonstrates it to be a member of the Fungi.