VISUAL PIGMENTS OF RODS AND CONES IN A HUMAN RETINA
VISUAL PIGMENTS OF RODS AND CONES IN A HUMAN RETINA
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DOI：
10.1113/jphysiol.1980.sp013097
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发表时间：
1980-01-01
影响因子：
5.5
通讯作者：
DARTNALL, HJA
中科院分区：
文献类型：
--
作者：
BOWMAKER, JK;DARTNALL, HJA
用显微分光光度计测量了人视网膜上单个视杆细胞和视锥细胞的色素，测量光束横向穿过孤立的外节。棒（n = 11）的平均吸收光谱在497.6 ± 0.01处具有峰。3.3 nm.平均横向吸光度为0.035 ±。0.007.确定了三类锥细胞。长波视锥（红视锥）具有562.8 ± λ max。4.7 nm（n = 19），平均横向吸光度为0.027 ± 0.025。0.005.中波视锥（绿色视锥）具有533.8 ± λ max。3.7 nm（n = 11），平均横向吸光度为0.032 ± 0.001nm。0.007.短波视锥（蓝色视锥）具有420.3 ± λ max。4.7 nm（n = 3），平均横向吸光度为0.037 ± 0.001nm。0.011.如果对视锥细胞的长度和感受器前吸收进行假设，则可以推导出与适当的π非常相似的视锥细胞的心理物理敏感性。W. S.斯泰尔斯如果假设视杆细胞的长度和感受器前的吸收，那么视杆细胞的心理物理敏感度比国际照明委员会的暗视敏感度函数要宽得多。
Microspectrophotometric measurements were made of the photopigments of individual rods and cones from the retina of a man. The measuring beam was passed transversely through the isolated outer segments. The mean absorbance spectrum for rods (n = 11) had a peak at 497.6 .+-. 3.3 nm. The mean transverse absorbance was 0.035 .+-. 0.007. Three classes of cones were identified. The long-wave cones (red cones) had a .lambda.max of 562.8 .+-. 4.7 nm (n = 19) with a mean transverse absorbance of 0.027 .+-. 0.005. The middle-wave cones (green cones) had a .lambda.max of 533.8 .+-. 3.7 nm (n = 11) with a mean transverse absorbance of 0.032 .+-. 0.007. The short-wave cones (blue cones) had a .lambda.max of 420.3 .+-. 4.7 nm (n = 3) with a mean transverse absorbance of 0.037 .+-. 0.011. If assumptions are made about the length of cones and pre-receptoral absorption, it is possible to derive psychophysical sensitivities for the cones closely resembling the appropriate .pi. mechanisms of W.S. Stiles. If assumptions are made about the length of rods and about pre-receptoral absorption, the psychophysical sensitivity derived for the rods is considerably broader than the C.I.E. [International Commission of Illumination] scotopic sensitivity function.