Mapping of novel regions of DNA gain and loss by comparative genomic hybridization in esophageal carcinoma in the Black and Colored populations of South Africa.
Mapping of novel regions of DNA gain and loss by comparative genomic hybridization in esophageal carcinoma in the Black and Colored populations of South Africa.
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发表时间：
1999-04
期刊：
影响因子：
11.2
通讯作者：
L. Plessis;E. Dietzsch;M. Gele;N. Roy;P. V. Helden;M. Parker;D. Mugwanya;M. D. Groot;M. P. Marx-M.-P
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作者：
L. Plessis;E. Dietzsch;M. Gele;N. Roy;P. V. Helden;M. Parker;D. Mugwanya;M. D. Groot;M. P. Marx-M.-P
食管癌（EC）是南非黑人男性癌症死亡的主要原因。虽然以前已经发现在这种癌症中有几个癌基因和肿瘤抑制基因发生了改变，但仍有许多新的基因有待鉴定。为了确定这些未知基因的染色体位置，我们分析了29个南非EC患者的DNA比较基因组杂交。染色体1 p（52%）、4p（52%）、18 q（48%）、19 p（52%）、19 q（55%）和22 q（41%）的丢失频率较高。在1 q（41%），2 q（52%），3q（72%），5 p（31%），7 p（48%），7 q（45%），8 q（55%）和Xq（69%）检测到最常见的增益。在2 q24 -33、6p21.1-q14、7 p12-q21、7q11.2-31、8 q22 -24、8 q13-qter、13 q21 -34和13 q32 -34检测到高水平扩增。目前的比较基因组杂交研究开辟了对这些特定染色体区域进行额外靶向研究的途径，以确定不同地理区域食管癌特定亚型易感性较高的特定基因。8 p和Xp在男性肿瘤中的缺失率分别为28%和17%，这可能为EC发生率的性别偏向性提供了线索。
Esophageal cancer (EC) is the leading cause of cancer death in the Black male population in South Africa. Although several oncogenes and tumor suppressor genes have previously been found altered in this cancer, many novel genes remain to be identified. To identify the chromosomal location of these unknown genes, we have analyzed DNA of 29 South African EC patients by comparative genomic hybridization. Frequent loss occurred at chromosome 1p (52%), 4p (52%), 18q (48%), 19p (52%), 19q (55%), and 22q (41%). The most common gains were detected at 1q (41%), 2q (52%), 3q (72%), 5p (31%), 7p (48%), 7q (45%), 8q (55%), and Xq (69%). High level amplification was detected at 2q24-33, 6p21.1-q14, 7p12-q21, 7q11.2-31, 8q22-24, 8q13-qter, 13q21-34, and at 13q32-34. The present comparative genomic hybridization study opens the way for additional targeted studies on these particular chromosomal regions to identify the specific genes involved in the higher susceptibility to specific subtypes of esophageal carcinoma in different geographical regions. The loss of 8p (28%) and Xp (17%) in tumors of male individuals may provide clues to the basis of the sex-biased frequency of occurrence of EC favoring men.