MODE OF ACTION OF STREPTOLYDIGIN
MODE OF ACTION OF STREPTOLYDIGIN
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DOI：
10.1128/jb.99.1.151-155.1969
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发表时间：
1969-01-01
影响因子：
3.2
通讯作者：
WEISBLUM, B
中科院分区：
文献类型：
--
作者：
SIDDHIKOL, C;ERBSTOESZER, JW;WEISBLUM, B
链溶菌素和利福霉素抑制核糖核酸(RNA)聚合酶的催化功能。链溶菌素可以在反应开始后抑制聚合，而利福霉素只有在添加底物之前与RNA聚合酶预先孵育才有效。如果在检测系统中使用核苷三磷酸-焦磷酸交换反应，则对于这两种抗生素也观察到相同的关系。链溶菌素的抑制作用可通过进一步添加RNA聚合酶而可逆，但不能通过向检测系统中添加脱氧核糖核酸来逆转。
Streptolydigin and rifamycin inhibit the catalytic function of ribonucleic acid (RNA) polymerase. Streptolydigin can inhibit polymerization after the reaction has started, whereas rifamycin is effective only if it is preincubated with RNA polymerase prior to the addition of substrates. The same relationships are observed with respect to these two antibiotics if the nucleoside triphosphate-pyrophosphate exchange reaction is used in the assay system. The inhibitory effect of streptolydigin is reversible by further addition of RNA polymerase but not by addition of deoxyribonucleic acid to the assay system.