From marrow to brain: Expression of neuronal phenotypes in adult mice
From marrow to brain: Expression of neuronal phenotypes in adult mice
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DOI：
10.1126/science.290.5497.1775
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发表时间：
2000-12-01
期刊：
影响因子：
56.9
通讯作者：
Blau, HM
中科院分区：
文献类型：
--
作者：
Brazelton, TR;Rossi, FMV;Blau, HM
经血管内递送遗传标记的成年小鼠骨髓至经致死性辐照的正常成年宿主中后，在中枢神经系统中产生表达神经元蛋白（神经元表型）的供体衍生细胞。流式细胞术显示，在大脑中的供体来源的细胞与骨髓不同的特性的人口。单个细胞的共聚焦显微镜显示，脑切片中数百个骨髓来源的细胞表达神经元典型的基因产物（NeuN、200千道尔顿神经丝和III类β-微管蛋白），并且能够激活转录因子CAMP反应元件结合蛋白（CREB）。成人骨髓移植后1至6个月在成人脑中神经元表型的产生证明了具有潜在临床应用的成人组织的显著可塑性。
After intravascular delivery of genetically marked adult mouse bone marrow into Lethally irradiated normal adult hosts, donor-derived cells expressing neuronal proteins (neuronal phenotypes) developed in the central nervous system. Flow cytometry revealed a population of donor-derived cells in the brain with characteristics distinct from bone marrow. Confocal microscopy of individual cells showed that hundreds of marrow-derived cells in brain sections expressed gene products typical of neurons (NeuN, 200-kilodalton neurofilament, and class III beta -tubulin) and were able to activate the transcription factor CAMP response element-binding protein (CREB). The generation of neuronal phenotypes in the adult brain 1 to 6 months after an adult bone marrow transplant demonstrates a remarkable plasticity of adult tissues with potential clinical applications.