Reexamining the polyadenylation signal: were we wrong about AAUAAA?
Reexamining the polyadenylation signal: were we wrong about AAUAAA?
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DOI：
10.1016/s0303-7207(02)00044-8
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发表时间：
2002-04-25
影响因子：
4.1
通讯作者：
Redondo, JL
中科院分区：
文献类型：
--
作者：
MacDonald, CC;Redondo, JL
多聚腺苷酸化是大多数真核生物 mRNA 形成其 3' 末端的过程。长期以来，人们一直认为多聚腺苷酸化需要序列 AAUAAA，并且 90% 的 mRNA 在多聚腺苷酸添加位点的 30 个核苷酸内具有 AAUAAA。最近的研究在对 GenBank 和表达序列标签 (EST) 数据库中提供的序列进行计算机分析的帮助下，表明 AAUAAA 的实际发病率要低得多，可能低至 50-60%。生殖生物学家很早就认识到，哺乳动物雄性生殖细胞中的大量 mRNA 缺乏 AAUAAA，但在其他方面通常是聚腺苷酸化的。我们实验室最近的研究发现了一种新形式的必需聚腺苷酸化蛋白，tauCstF-64，它在男性生殖细胞中表达最高，在大脑中表达程度较小，我们认为它在生殖细胞中不依赖于 AAUAAA 的 mRNA 聚腺苷酸化中发挥着重要作用。 (C) 2002 Elsevier Science Ireland Ltd. 保留所有权利。
Polyadenylation is the process by which most eukaryotic mRNAs form their 3' ends. It was long held that polyadenylation required the sequence AAUAAA and that 90% of mRNAs had AAUAAA within 30 nucleotides of the site of poly(A) addition. More recent studies, aided by computer analysis of sequences made available in GenBank and expressed sequence tag (EST) databases, have suggested that the actual incidence of AAUAAA is much lower, perhaps as low as 50-60%. Reproductive biologists have long recognized that a large number of mRNAs in male germ cells of mammals lack AAUAAA but are otherwise normally polyadenylated. Recent research in our laboratory has uncovered a new form of an essential polyadenylation protein, tauCstF-64, that is most highly expressed in male germ cells, and to a smaller extent in the brain, and which we propose plays a significant role in AAUAAA-independent mRNA polyadenylation in germ cells. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.