Isolation of mesenchymal stromal/stem cells from small-volume umbilical cord blood units that do not qualify for the banking system.
Isolation of mesenchymal stromal/stem cells from small-volume umbilical cord blood units that do not qualify for the banking system.
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从不符合银行系统资格的小容量脐带血单位中分离间充质基质/干细胞。
DOI：
10.1007/s12185-015-1828-7
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发表时间：
2015
影响因子：
2.1
通讯作者：
Maekawa T (#Corresponding author).
中科院分区：
文献类型：
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作者：
Yoshioka S;#Miura Y;Iwasa M;Fujishiro A;Yao H;Miura M;Fukuoka M;Nakagawa Y;Yokota A;Hirai H;Ichinohe T;Takaori-Kondo A;Maekawa T (#Corresponding author).
间充质基质/干细胞（mesenchymal stromal/stem cells，MSCs）的临床应用已被广泛探索。在这项研究中，我们检查了新鲜捐献的脐带血（UCB）单位的可用性，这些单位不符合日本银行系统的移植条件，因为它们作为MSC来源的体积很小。使用了45个UCB装置。每个UCB单位的中位体积和单位有核细胞数分别为40 mL和5.39 × 108。从45个单位中的18个（40%）成功分离MSC。MSC分离率不受细胞处理方法或递送与细胞处理之间的间隔的影响。脐血单位体积和单个核细胞计数是MSC分离率的预测因素。选择体积≥54 mL、单个核细胞数≥1.28 × 108的脐血单位可有效分离MSC，MSC分离率> 70%。脐带血来源的间充质干细胞与骨髓来源的间充质干细胞在形态学、表面标志物表达和分化潜能方面相似，除了脂肪形成。我们的数据表明，目前由于体积不足而被丢弃的UCB单位应重新考虑作为使用完善的UCB银行系统的MSC来源。
The clinical application of mesenchymal stromal/stem cells (MSCs) has been extensively explored. In this study, we examined the availability of freshly donated umbilical cord blood (UCB) units that do not qualify for the Japanese banking system for transplantation because of their small volume as a source of MSCs. Forty-five UCB units were used. The median volume of each UCB unit and number of nucleated cells per unit were 40 mL and 5.39 × 108, respectively. MSCs were successfully isolated from 18 of 45 units (40 %). The MSC isolation rate was not affected by cell processing method or the interval between delivery and cell processing. The volume of the UCB unit and the mononuclear cell count were predictive factors of the MSC isolation rate. MSCs were effectively isolated by selecting UCB units with a volume of ≥54 mL and containing ≥1.28 × 108mononuclear cells, yielding a MSC isolation rate of >70 %. UCB-derived MSCs were similar to bone marrow-derived MSCs in terms of their morphology, surface marker expression, and differentiation potential, apart from adipogenesis. Our data indicate that UCB units that are currently discarded due to inadequate volume should be reconsidered as a source of MSCs using the well-established UCB banking system.