Polyketides from the Cultured Lichen Mycobiont of a Vietnamese Pyrenula sp.
Polyketides from the Cultured Lichen Mycobiont of a Vietnamese Pyrenula sp.
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DOI：
10.1021/np500143k
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发表时间：
2014-06-01
影响因子：
5.1
通讯作者：
Tanahashi, Takao
中科院分区：
文献类型：
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作者：
Le, Duy Hoang;Takenaka, Yukiko;Tanahashi, Takao
在添加10%蔗糖的麦芽-酵母提取物培养基上培养了一种产于越南的壳状核壳菌地衣孢子。对培养菌落的化学研究导致分离出八种新的烷基化十氢化萘型聚酮化合物（1-8）沿着分离出三种已知化合物。通过光谱和化学方法鉴定了这些化合物的结构。这是第一次从培养的地衣真菌中分离出这种类型的聚酮化合物。分离的聚酮化合物1和7显示出对哺乳动物DNA聚合酶α和β的抑制活性，IC 50值范围为8.1 - 19.5 μ M。化合物1显示出对HCT 116人结肠癌培养细胞系的细胞毒性作用，IC（50）值为6.4 ± 0.7 μ M。
A spore-derived mycobiont of a crustose Pyrenula sp. lichen collected in Vietnam was cultivated on a malt-yeast extract medium supplemented with 10% sucrose. Chemical investigation of the cultivated colonies led to the isolation of eight new alkylated decalin-type polyketides (1-8) along with three known compounds. The structures of these compounds were elucidated by spectroscopic and chemical means. This is the first instance of this type of polyketide being isolated from a cultured lichen mycobiont. The isolated polyketides 1 and 7 exhibited inhibitory activities against mammalian DNA polymerases alpha and beta with IC50 values ranging from 8.1 to 19.5 mu M. Compound 1 showed cytotoxic effects against the HCT116 human colon carcinoma cultured cell line with an IC(50)value of 6.4 +/- 0.7 mu M.