In vivo magnetic resonance imaging of single cells in mouse brain with optical validation
In vivo magnetic resonance imaging of single cells in mouse brain with optical validation
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DOI：
10.1002/mrm.20747
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发表时间：
2006-01-01
影响因子：
3.3
通讯作者：
Foster, PJ
中科院分区：
文献类型：
--
作者：
Heyn, C;Ronald, JA;Foster, PJ
在目前的工作中，我们证明，第一次，单细胞可以在小鼠大脑中检测到体内使用磁共振成像（MRI）。用超顺磁性氧化铁纳米颗粒标记细胞并注射到小鼠的循环中。在1.5 T临床MRI扫描仪上，使用优化的MR硬件和采用稳态采集（FIESTA）脉冲序列的快速成像，可以用高分辨率MRI可视化大脑微循环内捕获的单个细胞。单个细胞在MR图像上表现为离散的信号空洞。直接的光学验证提供了共配准信号空隙MRI与单细胞可视化使用高分辨率共聚焦显微镜。这项工作证明了MRI检测小动物单细胞的灵敏度，适用于从干细胞到癌细胞跟踪的广泛应用。
In the current work we demonstrate, for the first time, that single cells can be detected in mouse brain in vivo using magnetic resonance imaging (MRI). Cells were labeled with superparamagnetic iron oxide nanoparticles and injected into the circulation of mice. Individual cells trapped within the microcirculation of the brain could be visualized with high-resolution MRI using optimized MR hardware and the fast imaging employing steady state acquisition (FIESTA) pulse sequence on a 1.5 T clinical MRI scanner. Single cells appear as discrete signal voids on MR images. Direct optical validation was provided by coregistering signal voids on MRI with single cells visualized using high-resolution confocal microscopy. This work demonstrates the sensitivity of MRI for detecting single cells in small animals for a wide range of application from stem cell to cancer cell tracking.