Static and dynamic compression modulate matrix metabolism in tissue engineered cartilage
Static and dynamic compression modulate matrix metabolism in tissue engineered cartilage
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DOI：
10.1016/s0736-0266(01)00160-7
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发表时间：
2002-07-01
影响因子：
2.8
通讯作者：
Ratcliffe, A
中科院分区：
文献类型：
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作者：
Davisson, T;Kunig, S;Ratcliffe, A
已知静态和动态压缩调节关节软骨的代谢。本研究的重点是确定压缩负荷对组织工程软骨结构中硫酸化糖胺聚糖（S-GAG）和蛋白质代谢的影响。将Carbohydrate构建体进行静态或动态压缩24小时，并用（SO 4）-S-35和H-3-脯氨酸进行放射性标记，以分别评估S-GAG和总蛋白的总合成和保留百分比。静态压缩在振幅为50抑制合成的S-GAG和蛋白质的35%和57%，分别。5%幅度的动态压缩对合成具有刺激作用，其依赖于静态偏移压缩幅度（10%或50%）和动态压缩频率（0.001或0.1 Hz）。因此，组织工程软骨表现出以与关节软骨观察到的类似的方式响应机械负荷的能力。因此，机械负载可能用于调节软骨组织的体外生长，可能促进适合于植入的功能性软骨组织的培养。(C)2002骨科研究学会。由爱思唯尔科技有限公司出版。保留所有权利。
Static and dynamic compression are known to modulate the metabolism of articular cartilage. The present study focussed on determining the effect of compressive loading on the metabolism of sulfated glycosaminoglycans (S-GAG) and protein in tissue engineered cartilage constructs. Cartilage constructs were subjected to static or dynamic compression for 24 h and radiolabeled with (SO4)-S-35 and H-3-proline to assess the total synthesis and percentage retention of S-GAG and total protein, respectively. Static compression at an amplitude of 50 suppressed the synthesis of both S-GAG and protein by 35% and 57%, respectively. Dynamic compression at an amplitude of 5% had stimualtory effects on synthesis that were dependent on the static offset compression amplitude (10% or 50%) and dynamic compression frequency (0.001 or 0.1 Hz). Thus, tissue engineered cartilage demonstrated the ability to respond to mechanical loading in a manner similar to that observed with articular cartilage. Mechanical loading may therefore potentially be used to modulate the growth of cartilaginous tissues in vitro, potentially facilitating the culture of functional cartilage tissues suitable for implantation. (C) 2002 Orthopaedic Research Society. Published by Elsevier Science Ltd. All rights reserved.