Synthesis of hypomethylated Epstein-Barr viral DNA is stimulated by dimethylsulfoxide treatment of lymphoblastoid cells.
Synthesis of hypomethylated Epstein-Barr viral DNA is stimulated by dimethylsulfoxide treatment of lymphoblastoid cells.
复制标题
二甲基亚砜处理类淋巴母细胞可刺激低甲基化 Epstein-Barr 病毒 DNA 的合成。
DOI：
10.1016/s0006-291x(81)80213-6
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发表时间：
1981
影响因子：
3.1
通讯作者：
Clough,W
中科院分区：
文献类型：
--
作者：
Larocca,D;Homisak,D;Clough,W
二甲亚砜先前被描述为细胞分化诱导剂，可诱导 Epstein-Barr 病毒 DNA 合成，但抑制 Epstein-Barr 病毒产生者淋巴母细胞系中宿主细胞 DNA 合成。病毒DNA的合成受到刺激，呈低甲基化状态，而细胞DNA则高度甲基化，且其合成受到二甲亚砜处理细胞的抑制。在处理的细胞中没有观察到 DNA 甲基化模式的改变。因此，二甲亚砜似乎选择性地刺激先前低甲基化 DNA 的合成，同时抑制该病毒产生细胞系内甲基化 DNA 的合成。
Dimethylsulfoxide, previously described as an inducer of cell differentiation, induced Epstein-Barr viral DNA synthesis but inhibited host cell DNA synthesis in an Epstein-Barr virus producer lymphoblastoid cell line. The viral DNA, whose synthesis was stimulated, was hypomethylated, in contrast to the cellular DNA, which was highly methylated and whose synthesis was inhibited by dimethylsulfoxide treatment of the cells. No alteration of DNA methylation patterns was observed in treated cells. Dimethylsulfoxide therefore appeared to selectively stimulate synthesis of previously hypomethylated DNA while inhibiting synthesis of methylated DNA within this virus-producing cell line.