Critical numbers of neural crest cells are required in the pathways from the neural tube to the foregut to ensure complete enteric nervous system formation
Critical numbers of neural crest cells are required in the pathways from the neural tube to the foregut to ensure complete enteric nervous system formation
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DOI：
10.1242/dev.017418
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发表时间：
2008-05-01
期刊：
影响因子：
4.6
通讯作者：
Burns, Alan J.
中科院分区：
文献类型：
--
作者：
Barlow, Amanda J.;Wallace, Adam S.;Burns, Alan J.
肠神经系统（ENS）主要来源于迷走神经嵴细胞（NCC），其产生于体节1-7的水平。为了了解NCC祖细胞库的大小和组成如何影响ENS发育，我们通过消融邻近体节3-6的神经管以产生无神经节肠来减少NCC的数量。然后，我们将鹌鹑神经管的不同体节长度回植到消融区域以确定“临界点”，从而有足够的祖细胞可用于完全ENS形成。将迷走神经管、骶神经管或躯干神经管的一个体节长度添加到具有邻近体节3-6的神经管消融的胚胎中，导致ENS沿整个肠沿着形成。虽然这些额外的细胞有助于祖细胞库，但不同轴向水平的鹌鹑NCC在形成ENS的能力方面保留了其固有的特性;迷走神经NCC形成了大部分ENS，骶骨NCC贡献了有限数量的ENS细胞，而躯干NCC对ENS没有贡献。由于发现迷走神经NCC的一个体节长度几乎包括整个ENS，我们切除所有迷走神经嵴，并将迷走神经管的一个体节长度从体节1或体节3水平回植到位于体节3位置的迷走区。第3体节的NCC沿着整个肠道形成ENS，而第1体节的NCC则没有。内在的差异，如增加的增殖能力，如在体外和体内证明的，似乎是体节3 NCC形成整个ENS的能力的基础。
The enteric nervous system (ENS) is mainly derived from vagal neural crest cells (NCC) that arise at the level of somites 1-7. To understand how the size and composition of the NCC progenitor pool affects ENS development, we reduced the number of NCC by ablating the neural tube adjacent to somites 3-6 to produce aganglionic gut. We then back-transplanted various somite lengths of quail neural tube into the ablated region to determine the 'tipping point', whereby sufficient progenitors were available for complete ENS formation. The addition of one somite length of either vagal, sacral or trunk neural tube into embryos that had the neural tube ablated adjacent to somites 3-6, resulted in ENS formation along the entire gut. Although these additional cells contributed to the progenitor pool, the quail NCC from different axial levels retained their intrinsic identities with respect to their ability to form the ENS; vagal NCC formed most of the ENS, sacral NCC contributed a limited number of ENS cells, and trunk NCC did not contribute to the ENS. As one somite length of vagal NCC was found to comprise almost the entire ENS, we ablated all of the vagal neural crest and back-transplanted one somite length of vagal neural tube from the level of somite 1 or somite 3 into the vagal region at the position of somite 3. NCC from somite 3 formed the ENS along the entire gut, whereas NCC from somite 1 did not. Intrinsic differences, such as an increased capacity for proliferation, as demonstrated in vitro and in vivo, appear to underlie the ability of somite 3 NCC to form the entire ENS.