INTACT MOTILITY AS A SALMONELLA-TYPHI INVASION-RELATED FACTOR
INTACT MOTILITY AS A SALMONELLA-TYPHI INVASION-RELATED FACTOR
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DOI：
10.1128/iai.56.8.1967-1973.1988
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发表时间：
1988-08-01
影响因子：
3.1
通讯作者：
YABUUCHI, E
中科院分区：
文献类型：
--
作者：
LIU, SL;EZAKI, T;YABUUCHI, E
伤寒沙门氏菌的侵袭性进行了调查。首先，我们将Tn 5导入野生型S.伤寒菌株GIFU 10007，并筛选非侵入性（Inv-）菌株的独立Tn 5插入突变体。在这项工作的前半部分，我们从1，338个独立的Tn 5突变体中获得了4个Inv-菌株。这四个是无鞭毛（弗拉-），monmotile（Mot-），或nonchemotactic（Che-）。然后，我们分离出更多的弗拉-、莫特-或切-突变体，并检查这些突变体的侵袭性。63个自发或Tn 5插入运动突变体，即，从野生型菌株GIFU 10007中独立地分离出弗拉-、Mot-或Che-;它们都是非侵入性的。从这些突变体中分离出的运动回复突变体显示出与亲本菌株相同的侵袭性。用P22介导的转导方法，将部分运动突变株（作为受体）与S.弗拉参考株进行杂交。在基因组上具有已知缺失位点的鼠伤寒沙门氏菌（作为供体）。对S.伤寒突变体几乎均匀地分配到S的三个鞭毛基因区域（区域I、II和III）。鼠伤寒。检查从转导测定获得的能动重组体的侵袭性。完整运动的恢复导致侵袭性的恢复。因此，我们认为完整的运动性是S.伤寒。Vi抗原与S.伤寒也进行了研究。具有完整运动性的Vi阴性突变体保持侵袭性，而所有63个Inv自发突变体或Tn 5突变体均为Vi阳性。因此，Vi抗原与S.伤寒。
Invasiveness of Salmonella typhi was investigated. At first, we introduced Tn5 into the chromosome of a wild-type S. typhi strain, GIFU 10007, and screened the independent Tn5 insertion mutants for noninvasive (Inv-) strains. During the first half of this work, we obtained 4 Inv- strains from 1,338 independent Tn5 mutants. The four were either nonflagellate (Fla-), monmotile (Mot-), or nonchemotactic (Che-). We then isolated more Fla-, Mot-, or Che- mutants and examined the invasiveness of these mutants. Sixty-three spontaneous or Tn5 insertion motility mutants, i.e., Fla-, Mot-, or Che-, were independently isolated from the wild-type strain GIFU 10007; all of them were noninvasive. Motile revertants isolated from some of these mutants showed the same invasiveness as the parent strain. P22-mediated transductional crosses were carried out between some of the motility mutants (as the recipients) and the Fla- reference strains of S. typhimurium with known deletion sites on the genome (as the donors). The mutational sites of the S. typhi mutants were assigned almost evenly to the three flagellar gene regions (regions I, II, and III) of S. typhimurium. The invasiveness of the motile recombinants obtained from the transduction assays was examined. The restoration of intact motility resulted in the restoration of invasiveness. Thus, we conclude that intact motility is an invasion-related factor of S. typhi. The relationship of Vi antigen to the invasiveness of S. typhi was also studied. Vi-negative mutnts with intact motility remained invasive, whereas all 63 Inv- spontaneous or Tn5 mutants were Vi positive. Therefore, Vi antigen was not related to the invasiveness of S. typhi.