The effect of nerve growth factor on corneal nerve regeneration and dry eye after LASIK
The effect of nerve growth factor on corneal nerve regeneration and dry eye after LASIK
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DOI：
10.1016/j.exer.2020.108428
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发表时间：
2021-01-09
影响因子：
3.4
通讯作者：
Hu, Liang
中科院分区：
文献类型：
--
作者：
Gong, Qianwen;Zhang, Shasha;Hu, Liang
干眼是屈光手术后最常见的并发症，尤其是激光原位角膜磨镶术（LASIK）后，在制作角膜瓣时可能会切断神经。神经生长因子 (NGF) 已被证明可以刺激角膜敏感性和神经再生，并且 NGF 已被建议作为干眼病 (DED) 的潜在治疗方法。因此，本研究旨在探讨与乙藻聚糖和生理盐水 (NS) 治疗相比，NGF 对 LASIK 术后角膜神经再生、敏感性和干眼症状的影响。 38 只接受 LASIK 手术的新西兰白兔被随机分为三组。每组均接受 NGF、hycosan 和 NS 治疗。用共聚焦显微镜测量神经密度以及角膜基底层和浅层基质神经的数量，并比较术前、术后1个月和3个月的结果。用感觉计评估角膜敏感性。记录泪膜破裂时间（TBUT）以检查是否有干眼症状。手术后三个月切除实验动物的整个角膜进行免疫组织化学分析。 LASIK 术后 1 个月和 3 个月，与海藻聚糖和 NS 治疗相比，NGF 治疗显着加速了基底下和浅层基质神经密度和数量的恢复（NGF 与海藻聚糖，P < 0.01；NGF 与 NS，P < 0.01）。与hycosan或NS治疗组相比，NGF组术后角膜敏感性恢复明显增强（P < 0.05）。此外，TBUT 数据显示 NGF 组术后 1 个月和 3 个月的时间显着延长（P < 0.05）。免疫荧光分析显示NGF组神经纤维数量多于hycosan组和NS组。综上所述，实验结果提示mNGF对促进角膜神经修复有明显作用，具有改善LASIK术后不同时期干眼症的潜力。
Dry eye is the most common complication after refractive surgery, especially after laser in situ keratomileusis (LASIK), in which nerves may be cut when making the corneal flap. Nerve growth factor (NGF) has been demonstrated to stimulate corneal sensitivity and nerve regeneration and NGF has been suggested as a potential treatment for dry eye disease (DED). Hence, this study aimed to investigate the effect of NGF on corneal nerve regeneration, sensitivity and dry eye symptoms after LASIK, compared to hycosan and normal saline (NS) treatments. Thirty-eight New Zealand white rabbits that underwent LASIK procedures were randomly assigned to three groups. Each group underwent NGF, hycosan, and NS treatment. The nerve densities and the number of corneal sub-basal and superficial stromal nerves were measured with confocal microscopy, and the results were compared before surgery and at one month and three months postoperatively. Corneal sensitivity was assessed with an esthesiometer. The tear breakup time (TBUT) was recorded to check for signs of dry eye. The whole corneas of the experimental animals were excised at three months after the surgery for immunohistochemically analysis. After LASIK, treatment with NGF significantly accelerated the recovery of sub-basal and superficial stromal nerve densities and the numbers, compared to hycosan and NS treatments at one month and three months postoperatively (NGF vs. hycosan, P < 0.01 each; NGF vs. NS, P < 0.01 each). The recovery of corneal sensitivity was significantly enhanced in the NGF group compared to the hycosan or NS treatment groups after surgery (P < 0.05). Also, the TBUT data showed a statistically significant longer time in the NGF group at one month, and three months postoperatively (P < 0.05). Immunofluorescence analysis showed the nerve fiber quantity of the NGF group was larger than in the hycosan and NS groups. Taken together, the experimental results suggested that mNGF had an obvious effect on promoting corneal nerve repairing and the potential to improve dry eye in different periods following LASIK.