Bombesin Receptor-Activated Protein (BRAP) Modulates NF-κB Activation in Bronchial Epithelial Cells by Enhancing HDAC Activity
Bombesin Receptor-Activated Protein (BRAP) Modulates NF-κB Activation in Bronchial Epithelial Cells by Enhancing HDAC Activity
复制标题
铃蟾肽受体激活蛋白 (BRAP) 通过增强 HDAC 活性来调节支气管上皮细胞中 NF-kappa B 的激活
DOI：
10.1002/jcb.25406
复制
发表时间：
2016-05-01
影响因子：
4
通讯作者：
Qu, Xiangping
中科院分区：
文献类型：
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作者：
Liu, Ying;Qin, Xiao-Qun;Qu, Xiangping
本课题组前期研究证实，由C6ORF89编码的bombesin receptor-activated protein （BRAP）在人气道上皮细胞中广泛表达，并可能在肺上皮的应激反应中发挥作用。在本研究中，我们证明了BRAP对培养的人支气管上皮细胞（HBECs）中NF-kappa B转录活性具有调节作用。BRAP基因转移过表达抑制了HBECs中基础的和诱导的NF-kappa B转录活性，而BRAP基因敲低则具有相反的作用。BRAP通过增强组蛋白去乙酰化酶（HDAC）活性来调节NF-kappa B活性。此外，BRAP可能会增加HDAC活性，从而通过其假定的c端结构域激活NF-kappa B。我们的研究表明BRAP蛋白是人气道上皮免疫和炎症反应的重要调节因子。(C) 2015 Wiley期刊公司
Our previous studies provided evidence that bombesin receptor-activated protein (BRAP), encoded by C6ORF89, is widely expressed in human airway epithelial cells and may play a role in the stress response of lung epithelia. In this study, we demonstrated that BRAP has a regulatory effect on NF-kappa B transcriptional activity in cultured human bronchial epithelial cells (HBECs). BRAP overexpression by gene transfer inhibited both basal and inducible NF-kappa B transcriptional activity in HBECs, whereas BRAP knockdown had the opposite effect. BRAP was shown to regulate NF-kappa B activity by enhancing histone deacetylase (HDAC) activity. In addition, BRAP might increase HDAC activity that leads to NF-kappa B activation via its putative C-terminal domain. Our study suggests that the BRAP protein is an important regulator of immune and inflammatory responses in the human airway epithelium. (C) 2015 Wiley Periodicals, Inc.