Identity of the F52F12.1 gene product in Caenorhabditis elegans as an organic cation transporter.
Identity of the F52F12.1 gene product in Caenorhabditis elegans as an organic cation transporter.
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秀丽隐杆线虫中 F52F12.1 基因产物作为有机阳离子转运蛋白的身份。
DOI：
10.1016/s0005-2736(99)00020-6
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Ganapathy,V
中科院分区：
文献类型：
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作者：
Wu,X;Fei,YJ;Huang,W;Chancy,C;Leibach,FH;Ganapathy,V
我们在这里描述的克隆和功能特性的有机阳离子转运体秀丽隐杆线虫（CeOCT1）。CeOCT1 cDNA全长1826 bp，编码568个氨基酸。oct1基因全长约3.2kb，由12个外显子组成。该基因的位置对应于染色体I上的F52F12.1基因座。该蛋白含有12个跨膜结构域。它与哺乳动物OCT具有显著的同源性。当在哺乳动物细胞中表达时，CeOCT 1诱导原型有机阳离子四乙基铵的转运。该底物的米氏常数为80±16 μM。CeOCT1的底物特异性很广。本研究首次报道了一个从C.优雅的。
We describe here the cloning and functional characterization of an organic cation transporter from Caenorhabditis elegans (CeOCT1). The CeOCT1 cDNA is 1826 bp long and codes for a protein of 568 amino acids. The oct1 gene is ∼3.2 kb in size and consists of 12 exons. The location of this gene corresponds to the F52F12.1 gene locus on chromosome I. The predicted protein contains 12 putative transmembrane domains. It exhibits significant homology to mammalian OCTs. When expressed in mammalian cells, CeOCT1 induces the transport of the prototypical organic cation tetraethylammonium. The Michaelis–Menten constant for this substrate is 80±16 μM. The substrate specificity of CeOCT1 is broad. This represents the first report on the cloning and functional characteristics of an organic cation transporter from C. elegans.