Quantitative immunolocalization of a P29 protein (GRA7), a new antigen of Toxoplasma gondii
Quantitative immunolocalization of a P29 protein (GRA7), a new antigen of Toxoplasma gondii
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DOI：
10.1177/002215549804601210
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发表时间：
1998-12-01
影响因子：
3.2
通讯作者：
Pinon, JM
中科院分区：
文献类型：
--
作者：
Bonhomme, A;Maine, GT;Pinon, JM
使用 P29 抗原特异性单克隆抗体 (5-241-178)，通过免疫金技术在薄片上检查弓形虫 P29 蛋白的超微结构定位。细胞外速殖子中 P29 蛋白的免疫定位证明该抗原存在于致密颗粒中。因此，我们已将这种 P29 抗原鉴定为定位于弓形虫致密颗粒的第七种蛋白质 (GRA7)。细胞内速殖子中的 P29 免疫定位证明了该抗原与寄生虫膜复合物、液泡内网络的管状元件以及寄生虫液泡膜的关联。我们的免疫标记数据表明，在速殖子侵入宿主细胞时，P29 (GRA7) 抗原从致密颗粒通过液泡内网络运输到寄生液泡膜。
Ultrastructural localization of a P29 protein of Toxoplasma gondii was examined on thin sections by an immunogold technique using a P29 antigen-specific monoclonal antibody (5-241-178). Immunolocalization of the P29 protein in extracellular tachyzoites demonstrated that this antigen was present in the dense granules. Thus, we have identified this P29 antigen as the seventh protein (GRA7) to be localized to the dense granules of T. gondii. P29 immunolocalization in intracellular tachyzoites demonstrated association of this antigen with the parasite membrane complex, tubular elements of the intravacuolar network, and with the parasitophorous vacuolar membrane. Our immunolabeling data suggest trafficking of the P29 (GRA7) antigen from the dense granule via the intravacuolar network to the parasitophorous vacuolar membrane on invasion of the tachyzoite into the host cell.