Humoral and cell-mediated immune responses of humans to inactivated influenza vaccine with or without QS21 adjuvant
Humoral and cell-mediated immune responses of humans to inactivated influenza vaccine with or without QS21 adjuvant
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DOI：
10.1016/j.vaccine.2007.01.073
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发表时间：
2007-04-30
期刊：
影响因子：
5.5
通讯作者：
Couch, R. B.
中科院分区：
文献类型：
--
作者：
Mbawuike, I.;Zang, Y.;Couch, R. B.
为了评估体液(抗体)和细胞免疫(CMI)反应，30名健康年轻人接种了含或不含QS21佐剂的流感灭活疫苗。QS21组接种部位疼痛和接种后肌痛较大。每种疫苗和抗原在2周和4周时，73-93%的受试者血清抗体升高，但两种疫苗的频率和平均滴度没有差异。甲型流感疫苗和乙型流感疫苗对感染细胞的T细胞毒性均无差异。在细胞毒性试验中，两种疫苗组在刺激3天后上清液中的干扰素-γ均有所增加，但在细胞毒性试验中7天未见增加；两种疫苗的量相似。为了进一步评估CMI，用每种疫苗株感染的细胞刺激剩余的PBMC过夜；所有受试者的颗粒酶B和干扰素-γ的斑点形成细胞(SFC)增加，在54个SFC测试中有51个增加。在Gran B测试中，QS21受试者的反应率略高，但在健康成年人中，没有发现QS21佐剂流感疫苗的明显免疫反应优势。(C)2007爱思唯尔有限公司。保留所有权利。
To evaluate humoral (antibody) and cell mediated immune (CMI) responses, 30 healthy young adults were either given inactivated influenza vaccine with or without QS21 adjuvant. Vaccination site pain and postvaccination myalgias were greater in the QS21 group. Serum antibody increases occurred in 73-93% of subjects for each vaccine and antigen at 2 weeks and 4 weeks but frequencies and mean titers for the two vaccines were not different. No differences in T cell cytotoxicity were detected for either vaccine for influenza A or B infected cells. IFN-gamma for both vaccine groups was increased in supernates after 3 days but not 7 days of stimulation in the cytotoxicity tests; amounts for the two vaccines were similar. To further evaluate CMI, remaining PBMCs were stimulated overnight with cells infected with each vaccine strain; an increase in spot forming cells (sfc) for Granzyme B and IFN-gamma was found for all subjects and in 51 of 54 sfc tests. A slightly higher response in the Gran B test for QS21 subjects was suggested, but no clear immune response advantage was identified among healthy adults for QS21 adjuvanted influenza vaccine. (c) 2007 Elsevier Ltd. All rights reserved.