Active membrane properties of rat neostriatal neurons in an in vitro slice preparation.
Active membrane properties of rat neostriatal neurons in an in vitro slice preparation.
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体外切片制剂中大鼠新纹状体神经元的活性膜特性。
DOI：
10.1007/bf00237018
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发表时间：
1985
影响因子：
2
通讯作者：
Kitai,ST
中科院分区：
文献类型：
--
作者：
Kita,H;Kita,T;Kitai,ST
用离体切片法研究了大鼠新纹状体神经元的活性膜特性。所有检查的神经元的静息膜电位超过50 mV，并产生幅度超过70 mV的动作电位。HRP细胞内标记的神经元形态特征表明，它们是中等棘状神经元。1.通过记录微电极的去极化电流注入产生慢去极化电位和重复动作电位，频率范围从小于10 Hz到超过300 Hz。长时程去极化电流可引起动作电位的适应性改变。2.去极化电流注射显示，纹状体神经元的膜电位去极化到静息电位时，有一个异常整流。一个可能的基础异常整流可能涉及失活的K-电导和缓慢的内向Ca-和/或Na-电流。3.局部电刺激诱发去极化突触后电位（DPSP），随后是持续时间长的小去极化。在一个双重刺激测试中，增强测试DPSP观察到在interstimulus时间间隔高达80 ms。强直后增强DPSP也被认为是在这些神经元。4.利用去极化电流注入、细胞内Cl−注入和Cl-电导阻断药物的测试表明，DPSP由EPSP和重叠的IPSP组成。5.无法最终确定DPSP之后的持久小去极化的性质。然而，现有的数据表明，缓慢的内向Cacurrent可能是负责这种反应。6.在某些神经元中，在局部刺激后观察到逆向反应。通过向神经元注入超极化电流或通过条件性局部刺激诱发的突触输入来阻断棘波侵入体细胞区域。这些发现可以解释以前的研究人员在体内制备中从新纹状体神经元获得逆向反应时遇到的困难。
The active membrane properties of rat neostriatal neurons have been studied in an in vitro slice preparation. All the neurons examined had resting membrane potentials of more than 50 mV and generated action potentials with amplitudes exceeding 70 mV. The morphological characteristics of the neurons identified by intracellular labeling with HRP indicated that they were medium spiny neurons. 1. Depolarizing current injection through the recording microelectrode generated slow depolarizing potentials and repetitive action potentials with frequencies ranging from less than 10 Hz to over 300 Hz. Adaptation of action potentials was observed when long duration depolarizing current was injected. 2. Depolarizing current injections revealed that the membrane of the striatal neuron had an anomalous rectification when the membrane potential was depolarized to the resting potential. A possible bases for the anomalous rectification might involve inactivation of K-conductance and slow inward Ca- and/or Na-currents. 3. Local electrical stimulation evoked depolarizing postsynaptic potentials (DPSPs) followed by long-lasting small depolarizations. In a double stimulation test, a potentiation of the test DPSP was observed at interstimulus time interval of up to 80 ms. Post-tetanic potentiation of DPSPs was also seen in these neurons. 4. Tests utilizing depolarizing current injection, intracellular Cl−injection, and Cl-conductance blocking drugs indicated that the DPSPs were composed of EPSPs and overlapping IPSPs. 5. The nature of the longlasting small depolarization succeeding the DPSPs could not be conclusively determined. However, available data suggest that the slow inward Cacurrent may be responsible for this response. 6. In some neurons, antidromic responses were observed following local stimulation. Spike invasion into the somatic region was blocked by an injection of hyperpolarizing current to the neuron or by synaptic inputs evoked by conditioning local stimulation. These findings may explain the difficulties encountered by previous investigators in obtaining antidromic responses from neostriatal neurons in in vivo preparation.