Endogenous hepadnaviruses, bornaviruses and circoviruses in snakes
Endogenous hepadnaviruses, bornaviruses and circoviruses in snakes
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DOI：
10.1098/rspb.2014.1122
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发表时间：
2014-09-22
影响因子：
4.7
通讯作者：
Schaack, S.
中科院分区：
文献类型：
--
作者：
Gilbert, C.;Meik, J. M.;Schaack, S.
我们报告的发现内源性病毒元件（EVES）肝DNA病毒科，Bornaviridae和Circoviridae的斑点响尾蛇，响尾蛇mitchellii，第一蝰蛇的草案全基因组序列组装可用。对组装草图的分析显示，在过去的5000万年里，来自三个病毒家族的基因组片段被插入到这种蛇的基因组中。正向基因座的跨物种PCR筛选和蟒蛇和眼镜王蛇基因组的计算扫描显示，圆环病毒最近整合（在最近的大约10年内）。10百万年），而博尔纳病毒和嗜肝DNA病毒分别整合了至少约13和约50 Ma。据我们所知，这是第一次报告的圆环病毒，博尔纳病毒和嗜肝DNA病毒在蛇和非逆转录病毒EVES在非鸟类爬行动物的第一个特征。我们的研究为了解这些宿主谱系中病毒的历史动态提供了一个窗口，并表明它们的进化涉及哺乳动物和爬行动物之间的多次宿主转换。
We report the discovery of endogenous viral elements (EVEs) from Hepadnaviridae, Bornaviridae and Circoviridae in the speckled rattlesnake, Crotalus mitchellii, the first viperid snake for which a draft whole genome sequence assembly is available. Analysis of the draft assembly reveals genome fragments from the three virus families were inserted into the genome of this snake over the past 50 Myr. Cross-species PCR screening of orthologous loci and computational scanning of the python and king cobra genomes reveals that circoviruses integrated most recently (within the last approx. 10 Myr), whereas bornaviruses and hepadnaviruses integrated at least approximately 13 and approximately 50 Ma, respectively. This is, to our knowledge, the first report of circo-, borna- and hepadnaviruses in snakes and the first characterization of non-retroviral EVEs in non-avian reptiles. Our study provides a window into the historical dynamics of viruses in these host lineages and shows that their evolution involved multiple host-switches between mammals and reptiles.