Mesenteric lymph flow in adult and aged rats
Mesenteric lymph flow in adult and aged rats
复制标题
DOI：
10.1152/ajpheart.00538.2011
复制
发表时间：
2011-11-01
影响因子：
4.8
通讯作者：
Gashev, Anatoliy A.
中科院分区：
文献类型：
--
作者：
Akl, Tony J.;Nagai, Takashi;Gashev, Anatoliy A.
首页--期刊主要分类--期刊细介绍--期刊题录与文摘内容成年和老年大鼠肠系膜淋巴流动的研究。Am J Physiol心圈Physiol 301：H1828-H1840,2011。2011年8月26日首次出版；DOI：10.1152/ajpheart.00538.2011。-该研究的目的是评估9岁和24个月大鼠的雄性Fischer-344大鼠的增龄相关变化、肠系膜淋巴管的收缩特性和体内淋巴流动。在活体大鼠MLV中，分别在100mM和100mM照射N-omega-硝基-L-精氨酸甲酯(L-NAME)前后，测定淋巴管直径、收缩幅度、收缩频率、泵流量分数、淋巴流速、壁面切应力和微小主动壁切应力负荷。这种变化与老年MLV增大有关，老年MLV经历的微小主动剪应力负荷比成年血管低得多。同时，体内老化MLV的泵血可能主要通过一氧化氮(NO)清除引起的收缩频率的增加而迅速增加到成年血管的水平。研究结果支持这样一种观点，即在老年MLV周围的老年组织中，一些尚未联系的淋巴收缩刺激代谢物的额外来源被NO释放抵消或阻止。对成人和老年MLV体内实验和基于血管的分离研究所获得的对照数据的比较分析清楚地表明，体外分离的淋巴管与体内淋巴管表现出相同的收缩特性。
Akl TJ, Nagai T, Cote GL, Gashev AA. Mesenteric lymph flow in adult and aged rats. Am J Physiol Heart Circ Physiol 301: H1828-H1840, 2011. First published August 26, 2011; doi:10.1152/ajpheart.00538.2011.-The objective of study was to evaluate the aging-associated changes, contractile characteristics of mesenteric lymphatic vessels (MLV), and lymph flow in vivo in male 9- and 24-mo-old Fischer-344 rats. Lymphatic diameter, contraction amplitude, contraction frequency, and fractional pump flow, lymph flow velocity, wall shear stress, and minute active wall shear stress load were determined in MLV in vivo before and after N-omega-nitro-L-arginine methyl ester hydrochloride (L-NAME) application at 100 mu M. The active pumping of the aged rat MLV in vivo was found to be severely depleted, predominantly through the aging-associated decrease in lymphatic contractile frequency. Such changes correlate with enlargement of aged MLV, which experienced much lower minute active shear stress load than adult vessels. At the same time, pumping in aged MLV in vivo may be rapidly increased back to levels of adult vessels predominantly through the increase in contraction frequency induced by nitric oxide (NO) elimination. Findings support the idea that in aged tissues surrounding the aged MLV, the additional source of some yet unlinked lymphatic contraction-stimulatory metabolites is counterbalanced or blocked by NO release. The comparative analysis of the control data obtained from experiments with both adult and aged MLV in vivo and from isolated vessel-based studies clearly demonstrated that ex vivo isolated lymphatic vessels exhibit identical contractile characteristics to lymphatic vessels in vivo.