Application of dipstick dye immunoassay (DDIA) kit for the diagnosis of schistosomiasis mekongi
Application of dipstick dye immunoassay (DDIA) kit for the diagnosis of schistosomiasis mekongi
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DOI：
10.1016/j.actatropica.2005.07.008
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发表时间：
2005-11-01
期刊：
影响因子：
2.7
通讯作者：
Bergquist, R
中科院分区：
文献类型：
--
作者：
Zhu, YC;Socheat, D;Bergquist, R
日本血吸虫抗体的试纸条染料免疫分析法（DDIA）是我国发展起来的一种检测日本血吸虫抗体的方法。该方法不仅快速、简便、价廉，而且特别适用于现场筛选。为了确定S.日本血吸虫抗原具有足够的交叉反应性，使得该测定也适用于诊断血吸虫。mekongi是一种基于S.在柬埔寨和老挝的湄公河血吸虫病非流行和流行地区的健康和感染人群中试验了日本血吸虫SEA。在对柬埔寨受试者进行检测时，灵敏度为97.1%，正确诊断了34名感染者中的33名。在老挝的敏感性为98.6%（69/70），在柬埔寨的非流行区的114名居民中无一人检测出阳性。与猫尾后睾吸虫感染者的交叉反应率为18.3%。结果支持了用S.日本血吸虫SEA抗原可以安全地用于湄公血吸虫病的诊断，但在解释从O.维韦里尼。(c)2005年由Elsevier B. V.出版
The dipstick dye immunoassay (DDIA), developed in China for the detection of antibodies against Schistosoma japonicum, relies on soluble egg antigen (SEA) labelled with a colloidal dye. This assay is not only rapid, simple and inexpensive, but also particularly useful for screening in the field. In order to determine whether S. japonicum antigens are sufficiently cross-reactive to make the assay applicable for the diagnosis also of S. mekongi a DDIA approach based on the S. japonicum SEA was tried in cohorts of healthy and infected people living in areas non-endemic and endemic with regard to schistosomiasis mekongi in Cambodia and Laos. A sensitivity of 97.1 % was recorded when testing Cambodian subjects, correctly diagnosing 33 out of 34 infected people. When the assay was applied in Laos, a sensitivity of 98.6% (69/70) was found. None of 114 residents living in a non-endemic area in Cambodia tested positive. A cross-reaction of 18.3% was found in patients infected with Opisthorchis viverrini. The results support the notion that the DDIA using S. japonicum SEA antigens can safely be implemented for the diagnosis of schistosomiasis mekongi, but care is needed in the interpretation of results obtained from areas that are co-endemic for O. viverrini. (c) 2005 Published by Elsevier B.V.