Re-evaluation of B lymphocyte lineage differentiation schemes.
Re-evaluation of B lymphocyte lineage differentiation schemes.
复制标题
B淋巴细胞谱系分化方案的重新评估。
DOI：
10.1007/978-3-642-57276-0_9
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发表时间：
2000
影响因子：
--
通讯作者：
Kouro,T
中科院分区：
文献类型：
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作者：
Kincade,PW;Payne,KJ;Tudor,KS;Yamashita,Y;Medina,KL;Rossi,MI;Kouro,T
造血干细胞的分化模型对于实验血液学家来说就像代谢路径图对于生物化学家一样。在预测B和T淋巴细胞生成的关键步骤的图表的背景下，考虑基因打靶和其他实验的结果是非常有用的。针对人类细胞的类似方案允许将恶性细胞分配到特定的分化阶段，并解释免疫缺陷。多参数流式细胞术和商用单抗的实际进展进一步解释了大多数实验室墙上描绘的分化方案的流行。然而，有理由相信，这些模型并不是在所有方面都是准确或足够完整的。我们将简要叙述我们最近的研究如何迫使重新评估一些被广泛接受的小鼠B系分化的里程碑。
Differentiation models for hematopoietic stem cells are to experimental hematologists what metabolic pathway charts are to biochemists. It has been extremely useful to consider the results of gene targeting and other experiments within the context of diagrams predicting key steps in B and T lymphopoiesis. Comparable schemes for human cells allow assignment of malignant cells to particular differentiation stages and interpretation of immunodeficiencies. Practical advances in multi-parameter flow cytometry and commercially available monoclonal antibodies further account for the popularity of differentiation schemes depicted on the walls of most laboratories. However, there is reason to believe that these models are not accurate or sufficiently complete in all respects. We will briefly recount how our recent studies forced re-evaluation of some widely accepted milestones for murine B lineage differentiation.