Pseudo half-molecules of the ABC transporter, COMATOSE, bind Pex19 and target to peroxisomes independently but are both required for activity.
Pseudo half-molecules of the ABC transporter, COMATOSE, bind Pex19 and target to peroxisomes independently but are both required for activity.
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ABC 转运蛋白 COMATOSE 的伪半分子结合 Pex19 并独立靶向过氧化物酶体，但两者都是活性所必需的。
DOI：
10.1016/j.febslet.2012.05.065
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发表时间：
2012
期刊：
影响因子：
3.5
通讯作者：
Nyathi Y
中科院分区：
文献类型：
--
作者：
Nyathi Y
动物和真菌的过氧化物酶体ABC转运蛋白是二聚化形成功能性转运蛋白的“半尺寸”蛋白。然而，陆地植物的过氧化物酶体ABC转运蛋白被合成为代表融合的异源二聚体的单一多肽。COMATOSE（CTS; AtABCD 1）的N-和C-末端假半部分表达为单独的多肽，其在体外结合Pex 19并独立靶向酵母中的过氧化物酶体膜，在那里它们是稳定的但不起作用。当共表达时，假半部分是完全功能性的，如ATP酶活性和pxa 1 pxa 2 Δ突变体在油酸盐上生长的拯救所示。异二聚体不对称的功能意义进行了讨论。蛋白质相互作用的结构总结：PEX 19 - 1通过下拉结合CTS-N（查看相互作用）PEX 19 - 1通过下拉结合CTS-C（查看相互作用）PEX 19 - 2通过下拉结合CTS-N（查看相互作用）PEX 19 - 2通过下拉结合CTS-N（查看相互作用）
Peroxisomal ABC transporters of animals and fungi are “half-size” proteins which dimerise to form a functional transporter. However, peroxisomal ABC transporters of land plants are synthesised as a single polypeptide which represents a fused heterodimer. The N- and C-terminal pseudo-halves of COMATOSE (CTS; AtABCD1) were expressed as separate polypeptides which bound Pex19 in vitro and targeted independently to the peroxisome membrane in yeast, where they were stable but not functional. When co-expressed, the pseudo-halves were fully functional as indicated by ATPase activity and rescue of the pxa1pxa2Δ mutant for growth on oleate. The functional significance of heterodimer asymmetry is discussed. STRUCTURED SUMMARY OF PROTEIN INTERACTIONS: PEX19-1binds to CTS-N by pull down (View Interaction) PEX19-1binds to CTS-C by pull down (View Interaction) PEX19-2binds to CTS-N by pull down (View Interaction) PEX19-2binds to CTS-N by pull down (View Interaction)