Identification of discrete classes of small nucleolar RNA featuring different ends and RNA binding protein dependency.
Identification of discrete classes of small nucleolar RNA featuring different ends and RNA binding protein dependency.
复制标题
DOI：
10.1093/nar/gku664
复制
发表时间：
2014-09
影响因子：
14.9
通讯作者：
Scott MS
中科院分区：
文献类型：
--
作者：
Deschamps-Francoeur G;Garneau D;Dupuis-Sandoval F;Roy A;Frappier M;Catala M;Couture S;Barbe-Marcoux M;Abou-Elela S;Scott MS
小核仁RNA （Small nucleolar RNA, snoRNAs）是最早发现和研究最广泛的小非编码RNA。然而，大多数研究集中在指导核糖体RNA修饰的一小部分snorna上。在本研究中，我们注释了所有box C/D snorna在正常和癌细胞系中独立于其功能的表达模式。结果表明，C/D snorna以两种不同的形式表达，其末端相对于盒C和D以及其末端茎长不同。这两种形式都在癌细胞系中过表达，但表现出保守的末端分布。令人惊讶的是，长形式比短形式更依赖于核心snoRNP蛋白NOP58的表达，NOP58被认为是C/D snoRNA产生所必需的。相反，短形式的子集依赖于剪接因子RBFOX2。对这两种形式的潜在二级结构的分析表明，结合NOP58所需的k-turn基序在短形式中不太稳定，因此不太可能成熟为标准的snoRNP。综合这些数据表明，C/D snorna至少分为两组，具有不同的成熟度和功能偏好。
Small nucleolar RNAs (snoRNAs) are among the first discovered and most extensively studied group of small non-coding RNA. However, most studies focused on a small subset of snoRNAs that guide the modification of ribosomal RNA. In this study, we annotated the expression pattern of all box C/D snoRNAs in normal and cancer cell lines independent of their functions. The results indicate that C/D snoRNAs are expressed as two distinct forms differing in their ends with respect to boxes C and D and in their terminal stem length. Both forms are overexpressed in cancer cell lines but display a conserved end distribution. Surprisingly, the long forms are more dependent than the short forms on the expression of the core snoRNP protein NOP58, thought to be essential for C/D snoRNA production. In contrast, a subset of short forms are dependent on the splicing factor RBFOX2. Analysis of the potential secondary structure of both forms indicates that the k-turn motif required for binding of NOP58 is less stable in short forms which are thus less likely to mature into a canonical snoRNP. Taken together the data suggest that C/D snoRNAs are divided into at least two groups with distinct maturation and functional preferences.
登录
查看更多内容
影响因子：
4.5
作者：
Huang, ZP;Zhou, H;Qu, LH
通讯作者：
Qu, LH
影响因子：
16
作者：
Hirose, T;Shu, MD;Steitz, JA
通讯作者：
Steitz, JA
影响因子：
20.3
作者：
Jima, Dereje D.;Zhang, Jenny;Dave, Sandeep S.
通讯作者：
Dave, Sandeep S.
DOI：
10.1073/pnas.231490998
发表时间：
2001-11-06
影响因子：
11.1
作者：
Hirose, T;Steitz, JA
通讯作者：
Steitz, JA
影响因子：
5.3
作者：
Caffarelli, E;Losito, M;Bozzoni, I
通讯作者：
Bozzoni, I