TagDust--a program to eliminate artifacts from next generation sequencing data.
TagDust--a program to eliminate artifacts from next generation sequencing data.
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DOI:
10.1093/bioinformatics/btp527
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发表时间:
2009-11-01
期刊:
影响因子:
--
通讯作者:
Daub CO
中科院分区:
文献类型:
--
作者:
Lassmann T;Hayashizaki Y;Daub CO
Motivation: Next-generation parallel sequencing technologies produce large quantities of short sequence reads. Due to experimental procedures various types of artifacts are commonly sequenced alongside the targeted RNA or DNA sequences. Identification of such artifacts is important during the development of novel sequencing assays and for the downstream analysis of the sequenced libraries. Results: Here we present TagDust, a program identifying artifactual sequences in large sequencing runs. Given a user-defined cutoff for the false discovery rate, TagDust identifies all reads explainable by combinations and partial matches to known sequences used during library preparation. We demonstrate the quality of our method on sequencing runs performed on Illumina's Genome Analyzer platform. Availability: Executables and documentation are available from http://genome.gsc.riken.jp/osc/english/software/. Contact: timolassmann@gmail.com
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影响因子:
14.9
作者:
Lassmann T;Frings O;Sonnhammer EL
通讯作者:
Sonnhammer EL
影响因子:
5.8
作者:
White, James Robert;Roberts, Michael;Pop, Mihai
通讯作者:
Pop, Mihai
DOI:
10.1111/j.2517-6161.1995.tb02031.x
发表时间:
1995-01-01
影响因子:
5.8
作者:
BENJAMINI, Y;HOCHBERG, Y
通讯作者:
HOCHBERG, Y
影响因子:
4.3
作者:
Zhang ZD;Rozowsky J;Snyder M;Chang J;Gerstein M
通讯作者:
Gerstein M