DEVELOPMENTAL-CHANGES OF SYNAPSIN-I SUBCELLULAR-LOCALIZATION IN RAT CEREBELLAR NEURONS
DEVELOPMENTAL-CHANGES OF SYNAPSIN-I SUBCELLULAR-LOCALIZATION IN RAT CEREBELLAR NEURONS
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DOI：
10.1247/csf.15.329
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发表时间：
1990-12-01
影响因子：
1.5
通讯作者：
HIROKAWA, N
中科院分区：
文献类型：
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作者：
HARADA, A;SOBUE, K;HIROKAWA, N
突触素I是主要的突触蛋白之一，被认为与突触小泡有关，并在神经递质的释放中发挥调节作用。在成熟神经元中，它几乎只集中在突触前神经末梢。本文用免疫细胞化学方法，用抗突触素I抗体对大鼠小脑皮质发育过程中突触素I的亚细胞定位进行了研究，发现在大鼠小脑皮质发育过程中，突触素I暂时存在于未成熟内颗粒细胞的树突生长锥、苔藓纤维的轴突生长锥以及成熟的突触前终末中。此外，我们还发现，轴突和树突生长锥中的突触素I并不一定与小泡有关，而是与细胞质中的模糊丝状结构有关。为了在体内寻找突触素I的结构，我们采用了免疫金标记法后的快速冷冻、深度蚀刻技术。突触素I似乎因此连接突触小泡或将它们锚定在突触前终末的细胞骨架上。
Synapsin I, one of the major synaptic proteins, is thought to associate with synaptic vesicles and to play a regulatory role in neurotransmitter release. In mature neurons, it is concentrated almost exclusively in presynaptic nerve endings. Here, we studied the subcellular localization of synapsin I during the development of rat cerebellar cortices by immunocytochemistry, using anti-synapsin I antibodies and found that during the development of rat cerebellar cortices it tentatively exists in the dendritic growth cones of immature internal granule cells and in the axonal growth cones of mossy fibers as well as mature presynaptic endings. Also, we found that synapsin I, in the axonal and dendritic growth cones does not necessarily associate with vesicles, but rather with fuzzy filamentous structures in the cytoplasm. In search of the structure of synapsin I in vivo, we employed the quick-freeze, deep-etch technique after immunogold labelling. Synapsin I seems to thereby connect synaptic vesicles or anchor them to cytoskeletons in presynaptic endings.