IDENTIFICATION OF THE 4 HUMAN MALARIA PARASITE SPECIES IN FIELD SAMPLES BY THE POLYMERASE CHAIN-REACTION AND DETECTION OF A HIGH PREVALENCE OF MIXED INFECTIONS
IDENTIFICATION OF THE 4 HUMAN MALARIA PARASITE SPECIES IN FIELD SAMPLES BY THE POLYMERASE CHAIN-REACTION AND DETECTION OF A HIGH PREVALENCE OF MIXED INFECTIONS
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DOI：
10.1016/0166-6851(93)90050-8
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发表时间：
1993-04-01
影响因子：
1.5
通讯作者：
BROWN, KN
中科院分区：
文献类型：
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作者：
SNOUNOU, G;VIRIYAKOSOL, S;BROWN, KN
属和种特异性序列存在于四种人类疟疾寄生虫的小亚基核糖体RNA基因中。采用聚合酶链式反应（PCR）技术，设计了针对每种疟原虫的寡核苷酸引物对，用于检测每种疟原虫。相当于5毫升血液的DNA足以检测每个物种。分析了在泰国特拉德省疟疾诊所就诊的196名患者的血液样本。仅通过PCR产物的电泳分析来检测和鉴定寄生虫，已被证明比常规诊断显微镜更敏感和准确。通过PCR检测发现了高比例的混合种感染。讨论了对医学治疗和流行病学研究的影响。
Genus- and species-specific sequences are present within the small subunit ribosomal RNA genes of the four human malaria parasites. Oligonucleotide primer pairs specific to each species were designed for specific amplification by the Polymerase Chain Reaction (PCR), to detect each malaria species. DNA equivalent to 5 mul of blood was sufficient for the detection of each of the species. Blood samples obtained from 196 patients attending a malaria clinic in Trad province (Thailand) were analyzed. Detection and identification of the parasites, solely by electrophoretic analysis of the PCR products, has proven to be more sensitive and accurate than by routine diagnostic microscopy. A high proportion of mixed species infections were brought to light by the PCR assay. Implications for medical treatment and epidemiological studies are discussed.