Effect of protein adsorption layers and solution treatments on hydroxyapatite deposition on polystyrene plate surfaces in simulated body fluids
Effect of protein adsorption layers and solution treatments on hydroxyapatite deposition on polystyrene plate surfaces in simulated body fluids
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DOI：
10.1007/s10856-017-6003-7
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发表时间：
2017-12-01
影响因子：
3.7
通讯作者：
Hashizume, Mineo
中科院分区：
文献类型：
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作者：
Iijima, Kazutoshi;Iizuka, Ayako;Hashizume, Mineo
我们已经开发了一种方法，功能化聚苯乙烯（PS）细胞培养板与羟基磷灰石（HAp）通过蛋白质吸附层，如人血清白蛋白（HSA）在模拟体液（SBF）。为了研究该方法的通用性，在这项研究中，蛋白质吸附层对HAp在SBF中PS板表面上沉积的影响进行了评估。用交替浸泡法（ASP）预处理，然后用SBF孵育24 h，可使HAp在PS板上沉积，其吸附层为HSA、I型胶原、鸡蛋白色溶菌酶和酸性蛋白类似物聚L-谷氨酸，沉积行为与蛋白质的吸附能力和电荷状态有关。我们还证明，市售的组织培养物处理的PS（TCPS）使用相同的ASP和SBF工艺直接涂覆有骨样HAp。人间充质干细胞以与通过HSA吸附层制备的HAp-coated PS板的情况类似的方式粘附并拉伸在HAp-coated TCPS板上。结果表明，本发明的方法可用于制备可利用吸附蛋白的功能且可方便且低成本地获得的骨样HAps包被的细胞培养板。
We have developed a method to functionalize polystyrene (PS) cell culture plates with hydroxyapatite (HAp) via protein adsorption layers such as human serum albumin (HSA) in simulated body fluids (SBFs). In order to investigate the versatility the method, in this study the effect of protein adsorption layers on HAp deposition on PS plate surfaces in SBF was evaluated. Pretreatments with alternate soaking process (ASP) using solutions containing calcium ions and phosphate ions followed by incubation with SBF for 24 h resulted in HAp deposition on PS plates with adsorption layers of HSA, type I collagen, hen egg white lysozyme, and poly L-glutamic acid, an acidic protein analogue: the deposition behaviors were correlated with adsorption ability and charge state of proteins. We also demonstrated that commercially available tissue culture-treated PS (TCPS) were directly coated with bone-like HAp using the same ASP and SBF processes. Human mesenchymal stem cells adhered and stretched on the HAp-coated TCPS plates in a similar manner to the case of the HAp-coated PS plates prepared via HSA adsorption layers. The results indicate that the present methods are useful for preparing bone-like HAp-coated cell culture plates that can be utilized function of adsorbed proteins and that are obtainable conveniently and at low-cost.