Vitroprocines, new antibiotics against Acinetobacter baumannii, discovered from marine Vibrio sp. QWI-06 using mass-spectrometry-based metabolomics approach.
Vitroprocines, new antibiotics against Acinetobacter baumannii, discovered from marine Vibrio sp. QWI-06 using mass-spectrometry-based metabolomics approach.
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Vitroprocines 是一种针对鲍曼不动杆菌的新型抗生素，从海洋弧菌中发现。
DOI：
10.1038/srep12856
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发表时间：
2015
影响因子：
4.6
通讯作者：
Yang,Yu-Liang
中科院分区：
文献类型：
--
作者：
Liaw,Chih-Chuang;Chen,Pei-Chin;Shih,Chao-Jen;Tseng,Sung-Pin;Lai,Ying-Mi;Hsu,Chi-Hsin;Dorrestein,PieterC;Yang,Yu-Liang
需要一个强大的和方便的研究策略，结合国家的最先进的分析技术，有效地发现新的化合物从海洋微生物资源。在本研究中，我们从海洋细菌Vibriosp中鉴定了一系列氨基聚酮衍生物，vitroprocines A-J。QWI-06通过使用成像质谱和分子网络以及常规生物活性引导的分馏和分离的综合方法。提出了玻丙碱类抗菌药物对鲍曼不动杆菌的构效关系。此外，用13 C标记的前体进行的饲养实验表明，5′-磷酸吡哆醛依赖性机制参与了vitropocines的生物合成。阐明的氨基聚酮衍生物从一种海洋细菌的第一次展示了这种集成的代谢组学方法，以揭示海洋细菌的生物多样性的潜力。
A robust and convenient research strategy integrating state-of-the-art analytical techniques is needed to efficiently discover novel compounds from marine microbial resources. In this study, we identified a series of amino-polyketide derivatives, vitroprocines A-J, from the marine bacteriumVibriosp. QWI-06 by an integrated approach using imaging mass spectroscopy and molecular networking, as well as conventional bioactivity-guided fractionation and isolation. The structure-activity relationship of vitroprocines againstAcinetobacter baumanniiis proposed. In addition, feeding experiments with13C-labeled precursors indicated that a pyridoxal 5′-phosphate-dependent mechanism is involved in the biosynthesis of vitroprocines. Elucidation of amino-polyketide derivatives from a species of marine bacteria for the first time demonstrates the potential of this integrated metabolomics approach to uncover marine bacterial biodiversity.