Scrotal infrared digital thermography as a predictor of seasonal effects on sperm traits in Braford bulls
Scrotal infrared digital thermography as a predictor of seasonal effects on sperm traits in Braford bulls
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DOI：
10.1007/s00484-014-0847-z
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发表时间：
2015-03-01
影响因子：
3.2
通讯作者：
Lopes, Flavio Guiselli
中科院分区：
文献类型：
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作者：
Oliveira Menegassi, Silvio Renato;Jardim Barcellos, Julio Otavio;Lopes, Flavio Guiselli
本研究的目的是评估季节性影响的环境对精液质量的公牛，使用红外热成像。在研究开始时，对17头约24月龄布拉德福德公牛的精子样本进行了精子活力（M）、质量运动（MM）和活力（VIG）评价。使用红外FLIR T 300相机和Quick Report 1.2 SP2软件收集红外热成像图像和数据，以确定睾丸近端和远端极的温度，并评估睾丸温度梯度。采用SASA（R）的GLM方差分析和CORR程序分析了季节对生理、精液和气候变量的影响。以小时为间隔记录小气候因子，计算日平均温度和平均相对湿度，确定1年内每天的日温湿度指数（THI）。睾丸的温度梯度（TG）变化在秋季（4.5A ℃）、冬季（4.0A ℃）和春季（2.9A ℃）显著高于夏季（0.9A ℃）（P < 0.05）。眼地球仪温度冬季（27.6 A ℃）和秋季（26.8 A ℃）低于夏季（33.9 A ℃）和春季（31.1 A ℃）（P < 0.05）。夏季精液中MM、M、VIG的平均值分别为2.58、52.64、2.70，与其他季节相比，差异有极显著性（P < 0.01）。TG与THI呈负相关（-0.44; P < 0.05）。精浆指标中，MaD（-0.45; P < 0.05）和TD（-0.50; P < 0.01）与TG呈负相关。TG与M和VIG呈正相关，相关系数分别为0.36和0.35（P < 0.05）。我们得出的结论是，红外热成像可以用来评估睾丸温度梯度和精子的物理和定量方面的后果。
The aim of this study was to assess the seasonal effects of the environment on semen quality in bulls, using infrared thermography. Sperm motility (M), mass motion (MM), and vigor (VIG) were evaluated in sperm samples from 17 Bradford bulls aged approximately 24 months at the beginning of the study. Infrared thermography images and data were collected using an infrared FLIR T 300 camera and Quick Report 1.2 SP2 software to determine the temperature of the proximal and distal poles of the testis and to assess the testicular temperature gradient. The seasonal effects on physiological, seminal, and climatic variables were analyzed by the GLM ANOVA and CORR procedures using SASA (R). The microclimatic factors were recorded in hourly intervals, and the daily mean temperature and mean relative humidity were calculated to determine the daily temperature-humidity index (THI) every day for 1 year. The temperature gradient (TG) variations of the testes were significantly higher in the autumn (4.5 A degrees C), winter (4.0 A degrees C), and spring (2.9 A degrees C) compared to summer (0.9 A degrees C) (P < 0.05). Ocular globe temperatures were lower in the winter (27.6 A degrees C) and autumn (26.8 A degrees C) compared to summer (33.9 A degrees C) and spring (31.1 A degrees C) (P < 0.05). The average MM (2.58), M (52.64), and VIG (2.70) of the semen decreased in the summer compared to other seasons (P < 0.01). The TG was negatively correlated with THI (-0.44; P < 0.05). For the seminal variables, MaD (-0.45; P < 0.05) and TD (-0.50; P < 0.01) presented a negative correlation with TG. The TG had a positive correlation between M and VIG, which had values of 0.36 and 0.35, respectively (P < 0.05). We have concluded that infrared thermography can be used to assess the testicular temperature gradient and its consequences on physical and quantitative aspects of sperm.