Eugenol (an essential oil of clove) acts as an antibacterial agent against Salmonella typhi by disrupting the cellular membrane
Eugenol (an essential oil of clove) acts as an antibacterial agent against Salmonella typhi by disrupting the cellular membrane
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DOI：
10.1016/j.jep.2010.04.025
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发表时间：
2010-07-06
影响因子：
5.4
通讯作者：
Pandian, S. Karutha
中科院分区：
文献类型：
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作者：
Devi, K. Pandima;Nisha, S. Arif;Pandian, S. Karutha
研究目的：为评价丁香酚对伤寒沙门氏菌的抗菌活性及其作用机制，采用纸片扩散法、MIC、MBC、时程试验和pH敏感性试验检测丁香酚对伤寒沙门氏菌的抗菌活性。丁香酚的化学引诱特性通过趋化性实验进行了验证。通过结晶紫法、260 nm吸收物质释放量测定、SDS-PAGE、FT-IR、AFM和SEM等方法确定丁香酚的作用方式。结果：丁香酚在其MIC（0.0125%）和MBC（0.025%）下处理可降低细菌的存活率并导致细菌的完全抑制。丁香酚在60分钟内灭活伤寒沙门氏菌。丁香酚的化学引诱性质与在碱性pH下观察到的高抗菌活性相结合，有利于该化合物在体内给药时更有效地发挥作用。丁香酚增加膜的渗透性，通过结晶紫测定证明。260 nm吸收的胞内物质释放的测量、SDS-PAGE、SEM和AFM分析证实了丁香酚对细胞质膜的破坏作用。通过FT-IR光谱证实了丁香酚处理后膜中大分子的变形。结论：丁香酚对伤寒沙门氏菌的抗菌活性是由于丁香酚与细菌细胞膜的相互作用。(C)2010爱思唯尔爱尔兰有限公司版权所有。
Aim of the study: To evaluate the antibacterial activity of eugenol and its mechanism of bactericidal action against Salmonella typhi.Materials and methods: The antibacterial activity was checked by disc-diffusion method, MIC, MBC, time course assay and pH sensitivity assay. The chemo-attractant property of eugenol was verified by chemotaxis assay. The mode of action of eugenol was determined by crystal violet assay, measurement of release of 260 nm absorbing material, SDS-PAGE, FT-IR spectroscopy, AFM and SEM.Results: Treatment with eugenol at their MIC (0.0125%) and MBC (0.025%) reduced the viability and resulted in complete inhibition of the organism. Eugenol inactivated Salmonella typhi within 60 min exposure. The chemo-attractant property of eugenol combined with the observed high antibacterial activity at alkaline pH favors the fact that the compound can work more efficiently when given in vivo. Eugenol increased the permeability of the membrane, as evidenced by crystal violet assay. The measurement of release of 260 nm absorbing intracellular materials, SDS-PAGE, SEM and AFM analysis confirmed the disruptive action of eugenol on cytoplasmic membrane. The deformation of macromolecules in the membrane, upon treatment with eugenol was verified by FT-IR spectroscopy.Conclusion: The results suggest that the antibacterial activity of eugenol against Salmonella typhi is due to the interaction of eugenol on bacterial cell membrane. (C) 2010 Elsevier Ireland Ltd. All rights reserved.