FIBRONECTIN MATRIX DEPOSITION AND FIBRONECTIN RECEPTOR EXPRESSION IN HEALING AND NORMAL SKIN
FIBRONECTIN MATRIX DEPOSITION AND FIBRONECTIN RECEPTOR EXPRESSION IN HEALING AND NORMAL SKIN
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DOI：
10.1111/1523-1747.ep12876104
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发表时间：
1990-06-01
影响因子：
6.5
通讯作者：
CLARK, RAF
中科院分区：
文献类型：
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作者：
CLARK, RAF
在皮肤组织形成过程中，细胞与细胞外基质(ECM)之间发生了许多重要的相互作用。细胞与基质的相互作用依赖于细胞外基质受体的存在。许多细胞外基质受体，称为整合素，是由一条α和一条β链组成的异二聚体糖蛋白。含有β1或β3链的整合素是细胞外基质受体，而含有β2链的整合素是白细胞-细胞受体。我们使用猪皮肤创伤作为组织组织的范例，并用纤维连接蛋白和纤维连接蛋白(α5β1)受体的多克隆抗体来探测愈合伤口和邻近的正常皮肤。在再上皮化过程中，表皮穿过含有纤维连接蛋白的临时基质。迁移中的表皮细胞呈明亮的线状周边型表达纤维连接蛋白受体。在10天时，当再上皮化完成并重建基底膜时，纤维连接蛋白基质显著减少，纤维连接蛋白受体的表达仅限于基底细胞的基底外侧，与正常表皮一样。在5-d创面的移行表皮下，肉芽组织填满了80%的创面间隙。第5天创伤成纤维细胞不表达纤维连接蛋白或其他β-1整合素受体，呈随机定向，不含肌动蛋白束。第5天创伤成纤维细胞表面可见纤维连接蛋白纤维，但细胞间联系较少。第7天创伤成纤维细胞表达纤维连接蛋白受体，含有与收缩成纤维细胞表型一致的外周细胞质肌动蛋白束，并与相互连接的纤维连接蛋白纤维平行排列。伤口在7至10天内收缩。因此，迁移的表皮持续表达纤维连接蛋白受体。纤维连接蛋白受体在伤口收缩前由成纤维细胞表达。
During cutaneous tissue organization, numerous critical interactions occur between cells and the extracellular matrix (ECM). Cell-matrix interactions depend on the presence of ECM receptors. Many ECM receptors, known as integrins, are heterodimeric glycoproteins consisting of one α and one β chain. Integrins containing β1 or β3 chains are ECM receptors, whereas those containing β2 chains are leukocyte cell-cell receptors. We have used porcine cutaneous wounds as a paradigm for tissue organization and probed healing wounds and adjacent normal skin with polyclonal antibodies to fibronectin and fibronectin (α5β1) receptor. During re-epithelialization, the epidermis transits over a provisional matrix containing fibronectin. Migrating epidermal cells expressed fibronectin receptors in a bright linear peripheral pattern. At 10 days, when re-epithelialization was complete and the basement membrane was re-established, the fibronectin matrix was markedly reduced and fibronectin-receptor expression was limited to the basolateral aspect of basal cells, as observed in normal epidermis. Beneath the migrating epidermis in 5-d wounds, granulation tissue had filled 80% of the wound space. Day-5 wound fibroblasts did not express fibronectin nor other β1 integrin receptors, were randomly oriented, and contained no actin bundles. Fibronectin fibrils were assembled on the surfaces of day-5 wound fibroblasts but formed few linkages between cells. Day-7 wound fibroblasts expressed fibronectin receptors, contained peripheral cytoplasmic actin bundles consistent with a contractile fibroblast phenotype, and were coaligned across the wound in parallel array with interconnecting fibronectin fibrils. The wounds contracted between 7 and 10 days. Thus the migrating epidermis consistently expressed fibronectin receptors. Fibronectin receptors were expressed by fibroblasts just prior to wound contraction.